2ab3
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2ab3.gif|left|200px]] | [[Image:2ab3.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2ab3", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_2ab3| PDB=2ab3 | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Solution structures and characterization of HIV RRE IIB RNA targeting zinc finger proteins''' | '''Solution structures and characterization of HIV RRE IIB RNA targeting zinc finger proteins''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB3 OCA]. | |
==Reference== | ==Reference== | ||
Solution structures and characterization of human immunodeficiency virus Rev responsive element IIB RNA targeting zinc finger proteins., Mishra SH, Shelley CM, Barrow DJ Jr, Darby MK, Germann MW, Biopolymers. 2006 Nov;83(4):352-64. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16826557 16826557] | Solution structures and characterization of human immunodeficiency virus Rev responsive element IIB RNA targeting zinc finger proteins., Mishra SH, Shelley CM, Barrow DJ Jr, Darby MK, Germann MW, Biopolymers. 2006 Nov;83(4):352-64. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16826557 16826557] | ||
| - | [[Category: Escherichia coli]] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Darby, M K.]] | [[Category: Darby, M K.]] | ||
[[Category: Germann, M W.]] | [[Category: Germann, M W.]] | ||
[[Category: Mishra, S H.]] | [[Category: Mishra, S H.]] | ||
[[Category: Shelley, C M.]] | [[Category: Shelley, C M.]] | ||
| - | [[Category: | + | [[Category: Beta beta alpha]] |
| - | [[Category: | + | [[Category: Rreiib-tr]] |
| - | [[Category: | + | [[Category: Zinc finger protein]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:50:14 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 15:50, 3 May 2008
Solution structures and characterization of HIV RRE IIB RNA targeting zinc finger proteins
Overview
The Rev responsive element (RRE), a part of unspliced human immunodeficiency virus (HIV) RNA, serves a crucial role in the production of infectious HIV virions. The viral protein Rev binds to RRE and facilitates transport of mRNA to the cytoplasm. Inhibition of the Rev-RRE interaction disrupts the viral life cycle. Using a phage display protocol, dual zinc finger proteins (ZNFs) were generated that bind specifically to RREIIB at the high affinity Rev binding site. These proteins were further shortened and simplified, and they still retained their RNA binding affinity. The solution structures of ZNF29 and a mutant, ZNF29G29R, have been determined by nuclear magnetic resonance (NMR) spectroscopy. Both proteins form C(2)H(2)-type zinc fingers with essentially identical structures. RNA protein interactions were evaluated quantitatively by isothermal titration calorimetry, which revealed dissociation constants (K(d)'s) in the nanomolar range. The interaction with the RNA is dependent upon the zinc finger structure; in the presence of EDTA, RNA binding is abolished. For both proteins, RNA binding is mediated by the alpha-helical portion of the zinc fingers and target the bulge region of RREIIB-TR. However, ZNF29G29R exhibits significantly stronger binding to the RNA target than ZNF29; this illustrates that the binding of the zinc finger scaffold is amenable to further improvements.
About this Structure
Full crystallographic information is available from OCA.
Reference
Solution structures and characterization of human immunodeficiency virus Rev responsive element IIB RNA targeting zinc finger proteins., Mishra SH, Shelley CM, Barrow DJ Jr, Darby MK, Germann MW, Biopolymers. 2006 Nov;83(4):352-64. PMID:16826557 Page seeded by OCA on Sat May 3 18:50:14 2008
