2ayt
From Proteopedia
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[[Image:2ayt.gif|left|200px]] | [[Image:2ayt.gif|left|200px]] | ||
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'''The crystal structure of a protein disulfide oxidoreductase from aquifex aeolicus''' | '''The crystal structure of a protein disulfide oxidoreductase from aquifex aeolicus''' | ||
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[[Category: Rossi, M.]] | [[Category: Rossi, M.]] | ||
[[Category: Simone, G De.]] | [[Category: Simone, G De.]] | ||
| - | [[Category: | + | [[Category: Glutaredoxin]] |
| - | [[Category: | + | [[Category: Protein disulfide oxidoreductase]] |
| - | [[Category: | + | [[Category: Thioredoxin fold]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:38:21 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 16:38, 3 May 2008
The crystal structure of a protein disulfide oxidoreductase from aquifex aeolicus
Overview
A potential role in disulfide bond formation in the intracellular proteins of thermophilic organisms has recently been attributed to a new family of protein disulfide isomerase (PDI)-like proteins. Members of this family are characterized by a molecular mass of about 26kDa and by two Trx folds, each comprising a CXXC active site motif. We report on the functional and structural characterization of a new member of this family, which was isolated from the thermophilic bacterium Aquifex aeolicus (AaPDO). Functional studies have revealed the high catalytic efficiency of this enzyme in reducing, oxidizing and isomerizing disulfide bridges. Site-directed mutagenesis experiments have suggested that its two active sites have similar functional properties, i.e. that each of them imparts partial activity to the enzyme. This similarity was confirmed by the analysis of the enzyme crystal structure, which points to similar geometrical parameters and solvent accessibilities for the two active sites. The results demonstrated that AaPDO is the most PDI-like of all prokaryotic proteins so far known. Thus, further experimental studies on this enzyme are likely to provide important information on the eukaryotic homologue.
About this Structure
2AYT is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.
Reference
Insights on a new PDI-like family: structural and functional analysis of a protein disulfide oxidoreductase from the bacterium Aquifex aeolicus., Pedone E, D'Ambrosio K, De Simone G, Rossi M, Pedone C, Bartolucci S, J Mol Biol. 2006 Feb 10;356(1):155-64. Epub 2005 Dec 1. PMID:16364362 Page seeded by OCA on Sat May 3 19:38:21 2008
