2bh2

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[[Image:2bh2.gif|left|200px]]
[[Image:2bh2.gif|left|200px]]
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{{Structure
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|PDB= 2bh2 |SIZE=350|CAPTION= <scene name='initialview01'>2bh2</scene>, resolution 2.15&Aring;
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The line below this paragraph, containing "STRUCTURE_2bh2", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Fmu+Binding+Site+For+Chain+D'>AC1</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=FMU:5-FLUORO-5-METHYLURIDINE-5&#39;-MONOPHOSPHATE'>FMU</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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|GENE=
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|DOMAIN=
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{{STRUCTURE_2bh2| PDB=2bh2 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bh2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bh2 OCA], [http://www.ebi.ac.uk/pdbsum/2bh2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2bh2 RCSB]</span>
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}}
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'''CRYSTAL STRUCTURE OF E. COLI 5-METHYLURIDINE METHYLTRANSFERASE RUMA IN COMPLEX WITH RIBOSOMAL RNA SUBSTRATE AND S-ADENOSYLHOMOCYSTEINE.'''
'''CRYSTAL STRUCTURE OF E. COLI 5-METHYLURIDINE METHYLTRANSFERASE RUMA IN COMPLEX WITH RIBOSOMAL RNA SUBSTRATE AND S-ADENOSYLHOMOCYSTEINE.'''
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[[Category: Stroud, R M.]]
[[Category: Stroud, R M.]]
[[Category: 4fe-4]]
[[Category: 4fe-4]]
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[[Category: base flipping]]
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[[Category: Base flipping]]
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[[Category: base stacking]]
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[[Category: Base stacking]]
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[[Category: direct protein sequencing]]
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[[Category: Direct protein sequencing]]
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[[Category: general base]]
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[[Category: General base]]
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[[Category: iron-sulfur cluster]]
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[[Category: Iron-sulfur cluster]]
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[[Category: metal-binding]]
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[[Category: Metal-binding]]
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[[Category: methyltransferase]]
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[[Category: Methyltransferase]]
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[[Category: ob-fold]]
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[[Category: Ob-fold]]
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[[Category: product release]]
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[[Category: Product release]]
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[[Category: protein-rna complex]]
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[[Category: Protein-rna complex]]
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[[Category: rna modification]]
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[[Category: Rna modification]]
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[[Category: rna processing]]
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[[Category: Rna processing]]
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[[Category: ruma]]
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[[Category: Ruma]]
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[[Category: sam]]
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[[Category: Sam]]
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[[Category: substrate selectivity]]
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[[Category: Substrate selectivity]]
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[[Category: transferase]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:16:39 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:06:19 2008''
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Revision as of 17:16, 3 May 2008

Image:2bh2.gif

Template:STRUCTURE 2bh2

CRYSTAL STRUCTURE OF E. COLI 5-METHYLURIDINE METHYLTRANSFERASE RUMA IN COMPLEX WITH RIBOSOMAL RNA SUBSTRATE AND S-ADENOSYLHOMOCYSTEINE.


Overview

A single base (U1939) within E. coli 23S ribosomal RNA is methylated by its dedicated enzyme, RumA. The structure of RumA/RNA/S-adenosylhomocysteine uncovers the mechanism for achieving unique selectivity. The single-stranded substrate is "refolded" on the enzyme into a compact conformation with six key intra-RNA interactions. The RNA substrate contributes directly to catalysis. In addition to the target base, a second base is "flipped out" from the core loop to stack against the adenine of the cofactor S-adenosylhomocysteine. Nucleotides in permuted sequence order are stacked into the site vacated by the everted target U1939 and compensate for the energetic penalty of base eversion. The 3' hairpin segment of the RNA binds distal to the active site and provides binding energy that contributes to enhanced catalytic efficiency. Active collaboration of RNA in catalysis leads us to conclude that RumA and its substrate RNA may reflect features from the earliest RNA-protein era.

About this Structure

2BH2 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

A unique RNA Fold in the RumA-RNA-cofactor ternary complex contributes to substrate selectivity and enzymatic function., Lee TT, Agarwalla S, Stroud RM, Cell. 2005 Mar 11;120(5):599-611. PMID:15766524 Page seeded by OCA on Sat May 3 20:16:39 2008

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