2bit

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[[Image:2bit.gif|left|200px]]
[[Image:2bit.gif|left|200px]]
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{{Structure
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|PDB= 2bit |SIZE=350|CAPTION= <scene name='initialview01'>2bit</scene>, resolution 1.71&Aring;
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The line below this paragraph, containing "STRUCTURE_2bit", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Peptidylprolyl_isomerase Peptidylprolyl isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.2.1.8 5.2.1.8] </span>
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{{STRUCTURE_2bit| PDB=2bit | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bit OCA], [http://www.ebi.ac.uk/pdbsum/2bit PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2bit RCSB]</span>
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'''CRYSTAL STRUCTURE OF HUMAN CYCLOPHILIN D AT 1.7 A RESOLUTION'''
'''CRYSTAL STRUCTURE OF HUMAN CYCLOPHILIN D AT 1.7 A RESOLUTION'''
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[[Category: Stihle, M.]]
[[Category: Stihle, M.]]
[[Category: Thoma, R.]]
[[Category: Thoma, R.]]
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[[Category: cis-tran-isomerization]]
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[[Category: Cis-tran-isomerization]]
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[[Category: crystal engineering]]
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[[Category: Crystal engineering]]
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[[Category: human]]
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[[Category: Human]]
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[[Category: isomerase]]
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[[Category: Isomerase]]
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[[Category: mitochondrial protein]]
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[[Category: Mitochondrial protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:21:00 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:07:02 2008''
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Revision as of 17:21, 3 May 2008

Image:2bit.gif

Template:STRUCTURE 2bit

CRYSTAL STRUCTURE OF HUMAN CYCLOPHILIN D AT 1.7 A RESOLUTION


Overview

In the pharmaceutical industry, knowledge of the three-dimensional structure of a specific target facilitates the drug-discovery process. Despite possessing favoured analytical properties such as high purity and monodispersion in light scattering, some proteins are not capable of forming crystals suitable for X-ray analysis. Cyclophilin D, an isoform of cyclophilin that is expressed in the mitochondria, was selected as a drug target for the treatment of cardiac disorders. As the wild-type enzyme defied all attempts at crystallization, protein engineering on the enzyme surface was performed. The K133I mutant gave crystals that diffracted to 1.7 A resolution using in-house X-ray facilities and were suitable for soaking experiments. The crystals were very robust and diffraction was maintained after soaking in 25% DMSO solution: excellent conditions for the rapid analysis of complex structures including crystallographic fragment screening.

About this Structure

2BIT is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal engineering yields crystals of cyclophilin D diffracting to 1.7 A resolution., Schlatter D, Thoma R, Kung E, Stihle M, Muller F, Borroni E, Cesura A, Hennig M, Acta Crystallogr D Biol Crystallogr. 2005 May;61(Pt 5):513-9. Epub 2005, Apr 20. PMID:15858260 Page seeded by OCA on Sat May 3 20:21:00 2008

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