2bz2

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:2bz2.gif|left|200px]]
[[Image:2bz2.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 2bz2 |SIZE=350|CAPTION= <scene name='initialview01'>2bz2</scene>
+
The line below this paragraph, containing "STRUCTURE_2bz2", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND=
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY=
+
or leave the SCENE parameter empty for the default display.
-
|GENE=
+
-->
-
|DOMAIN=
+
{{STRUCTURE_2bz2| PDB=2bz2 | SCENE= }}
-
|RELATEDENTRY=
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bz2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bz2 OCA], [http://www.ebi.ac.uk/pdbsum/2bz2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2bz2 RCSB]</span>
+
-
}}
+
'''SOLUTION STRUCTURE OF NELF E RRM'''
'''SOLUTION STRUCTURE OF NELF E RRM'''
Line 30: Line 27:
[[Category: Schweimer, K.]]
[[Category: Schweimer, K.]]
[[Category: Wohrl, B M.]]
[[Category: Wohrl, B M.]]
-
[[Category: alternative splicing]]
+
[[Category: Alternative splicing]]
-
[[Category: nelf e]]
+
[[Category: Nelf e]]
-
[[Category: nuclear protein]]
+
[[Category: Nuclear protein]]
-
[[Category: phosphorylation]]
+
[[Category: Phosphorylation]]
-
[[Category: repeat]]
+
[[Category: Repeat]]
-
[[Category: repressor]]
+
[[Category: Repressor]]
-
[[Category: rna recognition motif]]
+
[[Category: Rna recognition motif]]
-
[[Category: rna-binding]]
+
[[Category: Rna-binding]]
-
[[Category: transcription]]
+
[[Category: Transcription]]
-
[[Category: transcription regulation]]
+
[[Category: Transcription regulation]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:59:52 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:13:55 2008''
+

Revision as of 17:59, 3 May 2008

Image:2bz2.gif

Template:STRUCTURE 2bz2

SOLUTION STRUCTURE OF NELF E RRM


Overview

The elongation of transcription of HIV RNA at the TAR (transactivation-response element) is highly regulated by positive and negative factors. The cellular negative transcription elongation factor NELF (negative elongation factor) was suggested to be involved in transcriptional regulation of HIV-1 (HIV type 1) by binding to the stem of the viral TAR RNA which is synthesized by cellular RNA polymerase II at the viral long terminal repeat. NELF is a heterotetrameric protein consisting of NELF A, B, C or the splice variant D, and E. In the present study, we determined the solution structure of the RRM (RNA-recognition motif) of the RNA-binding subunit NELF E and studied its interaction with the viral TAR RNA. Our results show that the separately expressed recombinant NELF E RRM has alpha-helical and beta-strand elements adopting a betaalphabetabetaalphabeta fold and is able to bind to TAR RNA. Fluorescence equilibrium titrations with fluorescently labelled double- and single-stranded oligoribonucleotides representing the TAR RNA stem imply that NELF E RRM binds to the single-stranded TAR RNAs with K(d) values in the low-micromolar range.

About this Structure

2BZ2 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structural studies on the RNA-recognition motif of NELF E, a cellular negative transcription elongation factor involved in the regulation of HIV transcription., Rao JN, Neumann L, Wenzel S, Schweimer K, Rosch P, Wohrl BM, Biochem J. 2006 Dec 15;400(3):449-56. PMID:16898873 Page seeded by OCA on Sat May 3 20:59:52 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2bz2"
Personal tools