2c1o
From Proteopedia
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[[Image:2c1o.gif|left|200px]] | [[Image:2c1o.gif|left|200px]] | ||
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| - | + | {{STRUCTURE_2c1o| PDB=2c1o | SCENE= }} | |
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'''ENAIIHIS FAB FRAGMENT IN THE FREE FORM''' | '''ENAIIHIS FAB FRAGMENT IN THE FREE FORM''' | ||
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[[Category: Parkkinen, T.]] | [[Category: Parkkinen, T.]] | ||
[[Category: Rouvinen, J.]] | [[Category: Rouvinen, J.]] | ||
| - | [[Category: | + | [[Category: Antibody]] |
| - | [[Category: | + | [[Category: Ena11his antibody]] |
| - | [[Category: | + | [[Category: Enantioselective]] |
| - | [[Category: | + | [[Category: Fab fragment]] |
| - | [[Category: | + | [[Category: Finrozole]] |
| - | [[Category: | + | [[Category: Immune system]] |
| - | [[Category: | + | [[Category: Immunoglobulin domain]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 21:06:40 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 18:06, 3 May 2008
ENAIIHIS FAB FRAGMENT IN THE FREE FORM
Overview
Enantioselective antibodies can separate the enantiomers of a chiral compound in a highly specific manner. We have recently reported the cloning and applications of a recombinant Fab-fragment, ENA11His, in the enantioseparation of a drug candidate, finrozole, which contains two chiral centers. Here, the crystal structures of this enantioselective antibody Fab-fragment are determined in the absence of the hapten at a resolution of 2.75 A, and in the presence of the hapten at 2.05 A resolution. The conformation of the protein was found to be similar in both free and complex forms. The hapten molecule was tightly bound in a deep cleft between the light and heavy chains of the Fab-fragment. The complex structure also allowed us to describe the molecular basis for enantioselectivity and to deduce the absolute configurations of all the four different stereoisomers (a-d) of finrozole. The ENA11His antibody fragment selectively binds the SR (a) enantiomer from the racemic mixture of a and d-enantiomers, thus allowing separation from the pharmacologically most active RS enantiomer (d). In particular, Asp95 and Asn35 of the H-chain in the ENA11 His antibody seem to provide this specificity through hydrogen bonding.
About this Structure
2C1O is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Crystal structures of an enantioselective fab-fragment in free and complex forms., Parkkinen T, Nevanen TK, Koivula A, Rouvinen J, J Mol Biol. 2006 Mar 24;357(2):471-80. Epub 2006 Jan 3. PMID:16427081 Page seeded by OCA on Sat May 3 21:06:40 2008
