This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2cjs

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:2cjs.gif|left|200px]]
[[Image:2cjs.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 2cjs |SIZE=350|CAPTION= <scene name='initialview01'>2cjs</scene>, resolution 1.78&Aring;
+
The line below this paragraph, containing "STRUCTURE_2cjs", creates the "Structure Box" on the page.
-
|SITE= <scene name='pdbsite=AC1:Gol+Binding+Site+For+Chain+A'>AC1</scene>
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY=
+
or leave the SCENE parameter empty for the default display.
-
|GENE=
+
-->
-
|DOMAIN=
+
{{STRUCTURE_2cjs| PDB=2cjs | SCENE= }}
-
|RELATEDENTRY=
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cjs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cjs OCA], [http://www.ebi.ac.uk/pdbsum/2cjs PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2cjs RCSB]</span>
+
-
}}
+
'''STRUCTURAL BASIS FOR A MUNC13-1 HOMODIMER- MUNC13-1- RIM HETERODIMER SWITCH: C2-DOMAINS AS VERSATILE PROTEIN-PROTEIN INTERACTION MODULES'''
'''STRUCTURAL BASIS FOR A MUNC13-1 HOMODIMER- MUNC13-1- RIM HETERODIMER SWITCH: C2-DOMAINS AS VERSATILE PROTEIN-PROTEIN INTERACTION MODULES'''
Line 32: Line 29:
[[Category: Sudhof, T C.]]
[[Category: Sudhof, T C.]]
[[Category: Tomchick, D R.]]
[[Category: Tomchick, D R.]]
-
[[Category: alternative splicing]]
+
[[Category: Alternative splicing]]
-
[[Category: c2 domain]]
+
[[Category: C2 domain]]
-
[[Category: coiled coil]]
+
[[Category: Coiled coil]]
-
[[Category: exocytosis]]
+
[[Category: Exocytosis]]
-
[[Category: metal-binding]]
+
[[Category: Metal-binding]]
-
[[Category: munc13]]
+
[[Category: Munc13]]
-
[[Category: neurotransmitter release]]
+
[[Category: Neurotransmitter release]]
-
[[Category: neurotransmitter transport]]
+
[[Category: Neurotransmitter transport]]
-
[[Category: phorbol-ester binding]]
+
[[Category: Phorbol-ester binding]]
-
[[Category: protein-protein interaction]]
+
[[Category: Protein-protein interaction]]
-
[[Category: rim]]
+
[[Category: Rim]]
-
[[Category: synapse]]
+
[[Category: Synapse]]
-
[[Category: synaptosome]]
+
[[Category: Synaptosome]]
-
[[Category: transport]]
+
[[Category: Transport]]
-
[[Category: zinc]]
+
[[Category: Zinc]]
-
[[Category: zinc finger]]
+
[[Category: Zinc finger]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 22:18:46 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:22:30 2008''
+

Revision as of 19:18, 3 May 2008

Image:2cjs.gif

Template:STRUCTURE 2cjs

STRUCTURAL BASIS FOR A MUNC13-1 HOMODIMER- MUNC13-1- RIM HETERODIMER SWITCH: C2-DOMAINS AS VERSATILE PROTEIN-PROTEIN INTERACTION MODULES


Overview

C(2) domains are well characterized as Ca(2+)/phospholipid-binding modules, but little is known about how they mediate protein-protein interactions. In neurons, a Munc13-1 C(2)A-domain/RIM zinc-finger domain (ZF) heterodimer couples synaptic vesicle priming to presynaptic plasticity. We now show that the Munc13-1 C(2)A domain homodimerizes, and that homodimerization competes with Munc13-1/RIM heterodimerization. X-ray diffraction studies guided by nuclear magnetic resonance (NMR) experiments reveal the crystal structures of the Munc13-1 C(2)A-domain homodimer and the Munc13-1 C(2)A-domain/RIM ZF heterodimer at 1.44 A and 1.78 A resolution, respectively. The C(2)A domain adopts a beta-sandwich structure with a four-stranded concave side that mediates homodimerization, leading to the formation of an eight-stranded beta-barrel. In contrast, heterodimerization involves the bottom tip of the C(2)A-domain beta-sandwich and a C-terminal alpha-helical extension, which wrap around the RIM ZF domain. Our results describe the structural basis for a Munc13-1 homodimer-Munc13-1/RIM heterodimer switch that may be crucial for vesicle priming and presynaptic plasticity, uncovering at the same time an unexpected versatility of C(2) domains as protein-protein interaction modules, and illustrating the power of combining NMR spectroscopy and X-ray crystallography to study protein complexes.

About this Structure

2CJS is a Protein complex structure of sequences from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

Structural basis for a Munc13-1 homodimer to Munc13-1/RIM heterodimer switch., Lu J, Machius M, Dulubova I, Dai H, Sudhof TC, Tomchick DR, Rizo J, PLoS Biol. 2006 Jul;4(7):e192. PMID:16732694 Page seeded by OCA on Sat May 3 22:18:46 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2cjs"
Personal tools