2de6
From Proteopedia
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'''The reduced complex between oxygenase and ferredoxin in carbazole 1,9a-dioxygenase''' | '''The reduced complex between oxygenase and ferredoxin in carbazole 1,9a-dioxygenase''' | ||
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[[Category: Ashikawa, Y.]] | [[Category: Ashikawa, Y.]] | ||
[[Category: Nojiri, H.]] | [[Category: Nojiri, H.]] | ||
| - | [[Category: | + | [[Category: Carbazole 1,9a-dioxygenase]] |
| - | [[Category: | + | [[Category: Electron transfer complex]] |
| - | [[Category: | + | [[Category: Rieske non-heme iron oxygenase system]] |
| - | [[Category: | + | [[Category: Rieske-type ferredoxin]] |
| - | [[Category: | + | [[Category: Terminal oxygenase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 00:15:31 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 21:15, 3 May 2008
The reduced complex between oxygenase and ferredoxin in carbazole 1,9a-dioxygenase
Overview
Carbazole 1,9a-dioxygenase (CARDO), a member of the Rieske nonheme iron oxygenase system (ROS), consists of a terminal oxygenase (CARDO-O) and electron transfer components (ferredoxin [CARDO-F] and ferredoxin reductase [CARDO-R]). We determined the crystal structures of the nonreduced, reduced, and substrate-bound binary complexes of CARDO-O with its electron donor, CARDO-F, at 1.9, 1.8, and 2.0 A resolutions, respectively. These structures provide the first structure-based interpretation of intercomponent electron transfer between two Rieske [2Fe-2S] clusters of ferredoxin and oxygenase in ROS. Three molecules of CARDO-F bind to the subunit boundary of one CARDO-O trimeric molecule, and specific binding created by electrostatic and hydrophobic interactions with conformational changes suitably aligns the two Rieske clusters for electron transfer. Additionally, conformational changes upon binding carbazole resulted in the closure of a lid over the substrate-binding pocket, thereby seemingly trapping carbazole at the substrate-binding site.
About this Structure
2DE6 is a Protein complex structure of sequences from Janthinobacterium and Pseudomonas resinovorans. Full crystallographic information is available from OCA.
Reference
Electron transfer complex formation between oxygenase and ferredoxin components in Rieske nonheme iron oxygenase system., Ashikawa Y, Fujimoto Z, Noguchi H, Habe H, Omori T, Yamane H, Nojiri H, Structure. 2006 Dec;14(12):1779-89. PMID:17161368 Page seeded by OCA on Sun May 4 00:15:31 2008
