2df7
From Proteopedia
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'''Crystal structure of infectious bursal disease virus VP2 subviral particle''' | '''Crystal structure of infectious bursal disease virus VP2 subviral particle''' | ||
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[[Category: Wang, A H.]] | [[Category: Wang, A H.]] | ||
[[Category: Wang, M Y.]] | [[Category: Wang, M Y.]] | ||
| - | [[Category: | + | [[Category: Calcium]] |
| - | [[Category: | + | [[Category: Epitope]] |
| - | [[Category: | + | [[Category: Icosahedral capsid]] |
| - | [[Category: | + | [[Category: Immunogen]] |
| - | [[Category: | + | [[Category: Surface loop]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 00:18:34 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 21:18, 3 May 2008
Crystal structure of infectious bursal disease virus VP2 subviral particle
Overview
The structural protein VP2 of infectious bursal disease virus (IBDV) spontaneously forms a dodecahedral T=1 subviral particle (SVP), and is a primary immunogen of the virus. In this study, the structure of IBDV SVP was determined in a cubic crystal and refined to 2.6A resolution. It contains 20 independent VP2 subunits in a crystallographic asymmetric unit. Each subunit is folded mainly into a shell domain and a protrusion domain, both with the Swiss-roll topology, plus a small helical base domain. Three VP2 subunits constitute a tight trimer, which is the building block of IBDV (sub)viral particles. The structure revealed a calcium ion bound to three pairs of symmetry-related Asp31 and Asp174 to stabilize the VP2 trimer. Our results of treatment of SVP with EGTA, a Ca(2+)-chelating reagent, indicated that the metal-ion may be important not only in maintaining highly stable quaternary structure but also in regulating the swelling and dissociation of the icosahedral particles. A Ca(2+)-dependent assembly pathway was thus proposed, which involves further interactions between the trimers. The 20 independent subunits showed conformational variations, with the surface loops of the protrusion domain being the most diverse. These loops are targets of the neutralizing antibodies. Several common interactions between the surface loops were clearly observed, suggesting a possible major conformation of the immunogenic epitopes.
About this Structure
2DF7 is a Single protein structure of sequence from Infectious bursal disease virus. Full crystallographic information is available from OCA.
Reference
Crystal structure of infectious bursal disease virus VP2 subviral particle at 2.6A resolution: implications in virion assembly and immunogenicity., Lee CC, Ko TP, Chou CC, Yoshimura M, Doong SR, Wang MY, Wang AH, J Struct Biol. 2006 Jul;155(1):74-86. Epub 2006 Mar 31. PMID:16677827 Page seeded by OCA on Sun May 4 00:18:34 2008
