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2e7z

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[[Image:2e7z.jpg|left|200px]]
[[Image:2e7z.jpg|left|200px]]
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{{Structure
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|PDB= 2e7z |SIZE=350|CAPTION= <scene name='initialview01'>2e7z</scene>, resolution 1.26&Aring;
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The line below this paragraph, containing "STRUCTURE_2e7z", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=W:TUNGSTEN+ION'>W</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Deleted_entry Deleted entry], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.71 4.2.1.71] </span>
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or leave the SCENE parameter empty for the default display.
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{{STRUCTURE_2e7z| PDB=2e7z | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2e7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2e7z OCA], [http://www.ebi.ac.uk/pdbsum/2e7z PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2e7z RCSB]</span>
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'''Acetylene Hydratase from Pelobacter acetylenicus'''
'''Acetylene Hydratase from Pelobacter acetylenicus'''
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[[Category: Messerschmidt, A.]]
[[Category: Messerschmidt, A.]]
[[Category: Seiffert, G B.]]
[[Category: Seiffert, G B.]]
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[[Category: dmso reductase family]]
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[[Category: Dmso reductase family]]
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[[Category: iron-sulfur-cluster]]
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[[Category: Iron-sulfur-cluster]]
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[[Category: tungstoprotein]]
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[[Category: Tungstoprotein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 02:06:23 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:45:06 2008''
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Revision as of 23:06, 3 May 2008

Image:2e7z.jpg

Template:STRUCTURE 2e7z

Acetylene Hydratase from Pelobacter acetylenicus


Overview

The tungsten-iron-sulfur enzyme acetylene hydratase stands out from its class because it catalyzes a nonredox reaction, the hydration of acetylene to acetaldehyde. Sequence comparisons group the protein into the dimethyl sulfoxide reductase family, and it contains a bis-molybdopterin guanine dinucleotide-ligated tungsten atom and a cubane-type [4Fe:4S] cluster. The crystal structure of acetylene hydratase at 1.26 A now shows that the tungsten center binds a water molecule that is activated by an adjacent aspartate residue, enabling it to attack acetylene bound in a distinct, hydrophobic pocket. This mechanism requires a strong shift of pK(a) of the aspartate, caused by a nearby low-potential [4Fe:4S] cluster. To access this previously unrecognized W-Asp active site, the protein evolved a new substrate channel distant from where it is found in other molybdenum and tungsten enzymes.

About this Structure

2E7Z is a Single protein structure of sequence from Pelobacter acetylenicus. Full crystallographic information is available from OCA.

Reference

Structure of the non-redox-active tungsten/[4Fe:4S] enzyme acetylene hydratase., Seiffert GB, Ullmann GM, Messerschmidt A, Schink B, Kroneck PM, Einsle O, Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3073-7. PMID:17360611 Page seeded by OCA on Sun May 4 02:06:23 2008

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