2er8

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[[Image:2er8.gif|left|200px]]
[[Image:2er8.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2er8 |SIZE=350|CAPTION= <scene name='initialview01'>2er8</scene>, resolution 2.85&Aring;
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The line below this paragraph, containing "STRUCTURE_2er8", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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|GENE= LEU3 (32-103) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
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|DOMAIN=
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{{STRUCTURE_2er8| PDB=2er8 | SCENE= }}
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|RELATEDENTRY=[[2ere|2ERE]], [[2erg|2ERG]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2er8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2er8 OCA], [http://www.ebi.ac.uk/pdbsum/2er8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2er8 RCSB]</span>
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}}
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'''Crystal Structure of Leu3 DNA-binding domain complexed with a 12mer DNA duplex'''
'''Crystal Structure of Leu3 DNA-binding domain complexed with a 12mer DNA duplex'''
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[[Category: Fitzgerald, M X.]]
[[Category: Fitzgerald, M X.]]
[[Category: Marmorstein, R.]]
[[Category: Marmorstein, R.]]
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[[Category: zn(2)cys(6) binuclear cluster motif]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:01:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:52:44 2008''
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Revision as of 00:01, 4 May 2008

Image:2er8.gif

Template:STRUCTURE 2er8

Crystal Structure of Leu3 DNA-binding domain complexed with a 12mer DNA duplex


Overview

Gal4 is the prototypical Zn2Cys6 binuclear cluster transcriptional regulator that binds as a homodimer to DNA containing inverted CGG half-sites. Leu3, a member of this protein family, binds to everted (opposite polarity to inverted) CGG half-sites, and an H50C mutation within the Leu3 Zn2Cys6 binuclear motif abolishes its transcriptional repression function without impairing DNA binding. We report the X-ray crystal structures of DNA complexes with Leu3 and Leu3(H50C) and solution DNA binding studies of selected Leu3 mutant proteins. These studies reveal the molecular details of everted CGG half-site recognition, and suggest a role for the H50C mutation in transcriptional repression. Comparison with the Gal4-DNA complex shows an unexpected conservation in the DNA recognition mode of inverted and everted CGG half-sites, and points to a critical function of a linker region between the Zn2Cys6 binuclear cluster and dimerization regions in DNA binding specificity. Broader implications of these findings are discussed.

About this Structure

2ER8 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Structure of a Leu3-DNA complex: recognition of everted CGG half-sites by a Zn2Cys6 binuclear cluster protein., Fitzgerald MX, Rojas JR, Kim JM, Kohlhaw GB, Marmorstein R, Structure. 2006 Apr;14(4):725-35. PMID:16615914 Page seeded by OCA on Sun May 4 03:01:40 2008

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