2err
From Proteopedia
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'''NMR Structure of the RNA Binding Domain of Human Fox-1 in Complex with UGCAUGU''' | '''NMR Structure of the RNA Binding Domain of Human Fox-1 in Complex with UGCAUGU''' | ||
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[[Category: Allain, F H.]] | [[Category: Allain, F H.]] | ||
[[Category: Auweter, S D.]] | [[Category: Auweter, S D.]] | ||
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| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:02:33 2008'' | |
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Revision as of 00:02, 4 May 2008
NMR Structure of the RNA Binding Domain of Human Fox-1 in Complex with UGCAUGU
Overview
The Fox-1 protein regulates alternative splicing of tissue-specific exons by binding to GCAUG elements. Here, we report the solution structure of the Fox-1 RNA binding domain (RBD) in complex with UGCAUGU. The last three nucleotides, UGU, are recognized in a canonical way by the four-stranded beta-sheet of the RBD. In contrast, the first four nucleotides, UGCA, are bound by two loops of the protein in an unprecedented manner. Nucleotides U1, G2, and C3 are wrapped around a single phenylalanine, while G2 and A4 form a base-pair. This novel RNA binding site is independent from the beta-sheet binding interface. Surface plasmon resonance analyses were used to quantify the energetic contributions of electrostatic and hydrogen bond interactions to complex formation and support our structural findings. These results demonstrate the unusual molecular mechanism of sequence-specific RNA recognition by Fox-1, which is exceptional in its high affinity for a defined but short sequence element.
About this Structure
2ERR is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Molecular basis of RNA recognition by the human alternative splicing factor Fox-1., Auweter SD, Fasan R, Reymond L, Underwood JG, Black DL, Pitsch S, Allain FH, EMBO J. 2006 Jan 11;25(1):163-73. Epub 2005 Dec 15. PMID:16362037 Page seeded by OCA on Sun May 4 03:02:33 2008
