2ex3

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[[Image:2ex3.gif|left|200px]]
[[Image:2ex3.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2ex3 |SIZE=350|CAPTION= <scene name='initialview01'>2ex3</scene>, resolution 3.000&Aring;
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The line below this paragraph, containing "STRUCTURE_2ex3", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=PB:LEAD+(II)+ION'>PB</scene>, <scene name='pdbligand=UNK:UNKNOWN'>UNK</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= 2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10756 Bacillus phage phi29]), 3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10756 Bacillus phage phi29])
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-->
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|DOMAIN=
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{{STRUCTURE_2ex3| PDB=2ex3 | SCENE= }}
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|RELATEDENTRY=[[1xhx|1XHX]], [[1xhz|1XHZ]], [[1xi1|1XI1]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ex3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ex3 OCA], [http://www.ebi.ac.uk/pdbsum/2ex3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ex3 RCSB]</span>
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}}
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'''Bacteriophage phi29 DNA polymerase bound to terminal protein'''
'''Bacteriophage phi29 DNA polymerase bound to terminal protein'''
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[[Category: Vega, M de.]]
[[Category: Vega, M de.]]
[[Category: Wang, J.]]
[[Category: Wang, J.]]
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[[Category: dna polymerase: protein primer complex]]
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[[Category: Dna polymerase: protein primer complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:12:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:54:56 2008''
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Revision as of 00:12, 4 May 2008

Image:2ex3.gif

Template:STRUCTURE 2ex3

Bacteriophage phi29 DNA polymerase bound to terminal protein


Overview

The absolute requirement for primers in the initiation of DNA synthesis poses a problem for replicating the ends of linear chromosomes. The DNA polymerase of bacteriophage phi29 solves this problem by using a serine hydroxyl of terminal protein to prime replication. The 3.0 A resolution structure shows one domain of terminal protein making no interactions, a second binding the polymerase and a third domain containing the priming serine occupying the same binding cleft in the polymerase as duplex DNA does during elongation. Thus, the progressively elongating DNA duplex product must displace this priming domain. Further, this heterodimer of polymerase and terminal protein cannot accommodate upstream template DNA, thereby explaining its specificity for initiating DNA synthesis only at the ends of the bacteriophage genome. We propose a model for the transition from the initiation to the elongation phases in which the priming domain of terminal protein moves out of the active site as polymerase elongates the primer strand. The model indicates that terminal protein should dissociate from polymerase after the incorporation of approximately six nucleotides.

About this Structure

2EX3 is a Protein complex structure of sequences from Bacillus phage phi29. Full crystallographic information is available from OCA.

Reference

The phi29 DNA polymerase:protein-primer structure suggests a model for the initiation to elongation transition., Kamtekar S, Berman AJ, Wang J, Lazaro JM, de Vega M, Blanco L, Salas M, Steitz TA, EMBO J. 2006 Mar 22;25(6):1335-43. Epub 2006 Mar 2. PMID:16511564 Page seeded by OCA on Sun May 4 03:12:44 2008

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