2f5o

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[[Image:2f5o.gif|left|200px]]
[[Image:2f5o.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2f5o |SIZE=350|CAPTION= <scene name='initialview01'>2f5o</scene>, resolution 2.05&Aring;
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The line below this paragraph, containing "STRUCTURE_2f5o", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-formamidopyrimidine_glycosylase DNA-formamidopyrimidine glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.23 3.2.2.23] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2f5o| PDB=2f5o | SCENE= }}
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|RELATEDENTRY=[[2f5n|2F5N]], [[1l1t|1L1T]], [[1r2y|1R2Y]], [[2f5p|2F5P]], [[2f5q|2F5Q]], [[2f5s|2F5S]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f5o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f5o OCA], [http://www.ebi.ac.uk/pdbsum/2f5o PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2f5o RCSB]</span>
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}}
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'''MutM crosslinked to undamaged DNA sampling G:C base pair IC3'''
'''MutM crosslinked to undamaged DNA sampling G:C base pair IC3'''
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[[Category: Santos, W L.]]
[[Category: Santos, W L.]]
[[Category: Verdine, G L.]]
[[Category: Verdine, G L.]]
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[[Category: damage search]]
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[[Category: Damage search]]
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[[Category: disulfide crosslink]]
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[[Category: Disulfide crosslink]]
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[[Category: dna glycosylase]]
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[[Category: Dna glycosylase]]
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[[Category: dna repair]]
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[[Category: Dna repair]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:29:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:58:16 2008''
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Revision as of 00:29, 4 May 2008

Image:2f5o.gif

Template:STRUCTURE 2f5o

MutM crosslinked to undamaged DNA sampling G:C base pair IC3


Overview

DNA glycosylases must interrogate millions of base pairs of undamaged DNA in order to locate and then excise one damaged nucleobase. The nature of this search process remains poorly understood. Here we report the use of disulfide cross-linking (DXL) technology to obtain structures of a bacterial DNA glycosylase, MutM, interrogating undamaged DNA. These structures, solved to 2.0 angstrom resolution, reveal the nature of the search process: The protein inserts a probe residue into the helical stack and severely buckles the target base pair, which remains intrahelical. MutM therefore actively interrogates the intact DNA helix while searching for damage.

About this Structure

2F5O is a Single protein structure of sequence from Geobacillus stearothermophilus. Full crystallographic information is available from OCA.

Reference

Structure of a DNA glycosylase searching for lesions., Banerjee A, Santos WL, Verdine GL, Science. 2006 Feb 24;311(5764):1153-7. PMID:16497933 Page seeded by OCA on Sun May 4 03:29:24 2008

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OCA

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