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2fa1
From Proteopedia
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'''Crystal structure of PhnF C-terminal domain''' | '''Crystal structure of PhnF C-terminal domain''' | ||
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[[Category: Savchenko, A.]] | [[Category: Savchenko, A.]] | ||
[[Category: Skarina, T.]] | [[Category: Skarina, T.]] | ||
| - | [[Category: | + | [[Category: Apc5558]] |
| - | [[Category: | + | [[Category: Effector binding domain]] |
| - | [[Category: | + | [[Category: Mcsg]] |
| - | [[Category: | + | [[Category: Midwest center for structural genomic]] |
| - | [[Category: | + | [[Category: Pnhf]] |
| - | [[Category: | + | [[Category: Protein structure initiative]] |
| - | [[Category: | + | [[Category: Psi]] |
| - | [[Category: | + | [[Category: Regulator]] |
| - | [[Category: | + | [[Category: Transcription]] |
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Revision as of 00:38, 4 May 2008
Crystal structure of PhnF C-terminal domain
Overview
The crystal structure of Escherichia coli PhnF C-terminal domain (C-PhnF) was solved at 1.7 A resolution by the single wavelength anomalous dispersion (SAD) method. The PhnF protein belongs to the HutC subfamily of the large GntR transcriptional regulator family. Members of this family share similar N-terminal DNA-binding domains, but are divided into four subfamilies according to their heterogenic C-terminal domains, which are involved in effector binding and oligomerization. The C-PhnF structure provides for the first time the scaffold of this domain for the HutC subfamily, which covers about 31% of GntR-like regulators. The structure represents a mixture of alpha-helices and beta-strands, with a six-stranded antiparallel beta-sheet at the core. C-PhnF monomers form a dimer by establishing interdomain eight-strand beta-sheets that include core antiparallel and N-terminal two-strand parallel beta-sheets from each monomer. C-PhnF shares strong structural similarity with the chorismate lyase fold, which features a buried active site locked behind two helix-turn-helix loops. The structural comparison of the C-PhnF and UbiC proteins allows us to propose that a similar site in the PhnF structure is adapted for effector binding.
About this Structure
2FA1 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structural characterization of GntR/HutC family signaling domain., Gorelik M, Lunin VV, Skarina T, Savchenko A, Protein Sci. 2006 Jun;15(6):1506-11. Epub 2006 May 2. PMID:16672238 Page seeded by OCA on Sun May 4 03:38:58 2008
Categories: Escherichia coli | Single protein | Edwards, A M. | Gorelik, M. | Lunin, V V. | MCSG, Midwest Center for Structural Genomics. | Nocek, B P. | Savchenko, A. | Skarina, T. | Apc5558 | Effector binding domain | Mcsg | Midwest center for structural genomic | Pnhf | Protein structure initiative | Psi | Regulator | Transcription
