2gyb
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2gyb.gif|left|200px]] | [[Image:2gyb.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2gyb", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_2gyb| PDB=2gyb | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Structure of the 30S subunit of a SecM-stalled E. coli ribosome complex obtained by fitting atomic models for RNA and protein components into cryo-EM map EMD-1143''' | '''Structure of the 30S subunit of a SecM-stalled E. coli ribosome complex obtained by fitting atomic models for RNA and protein components into cryo-EM map EMD-1143''' | ||
| Line 27: | Line 24: | ||
[[Category: Frank, J.]] | [[Category: Frank, J.]] | ||
[[Category: Mitra, K.]] | [[Category: Mitra, K.]] | ||
| - | [[Category: | + | [[Category: Elongation arrest]] |
| - | [[Category: | + | [[Category: Nascent chain]] |
| - | [[Category: | + | [[Category: Polypeptide exit tunnel]] |
| - | [[Category: | + | [[Category: Protein-conducting channel]] |
| - | [[Category: | + | [[Category: Ribosome]] |
| - | [[Category: | + | [[Category: Rna world]] |
| - | [[Category: | + | [[Category: Secm]] |
| - | [[Category: | + | [[Category: Signal transduction]] |
| - | [[Category: | + | [[Category: Translocation]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:39:15 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 02:39, 4 May 2008
Structure of the 30S subunit of a SecM-stalled E. coli ribosome complex obtained by fitting atomic models for RNA and protein components into cryo-EM map EMD-1143
Overview
In E. coli, the SecM nascent polypeptide causes elongation arrest, while interacting with 23S RNA bases A2058 and A749-753 in the exit tunnel of the large ribosomal subunit. We compared atomic models fitted by real-space refinement into cryo-electron microscopy reconstructions of a pretranslocational and SecM-stalled E. coli ribosome complex. A cascade of RNA rearrangements propagates from the exit tunnel throughout the large subunit, affecting intersubunit bridges and tRNA positions, which in turn reorient small subunit RNA elements. Elongation arrest could result from the inhibition of mRNA.(tRNAs) translocation, E site tRNA egress, and perhaps translation factor activation at the GTPase-associated center. Our study suggests that the specific secondary and tertiary arrangement of ribosomal RNA provides the basis for internal signal transduction within the ribosome. Thus, the ribosome may itself have the ability to regulate its progression through translation by modulating its structure and consequently its receptivity to activation by cofactors.
About this Structure
2GYB is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Elongation arrest by SecM via a cascade of ribosomal RNA rearrangements., Mitra K, Schaffitzel C, Fabiola F, Chapman MS, Ban N, Frank J, Mol Cell. 2006 May 19;22(4):533-43. PMID:16713583 Page seeded by OCA on Sun May 4 05:39:15 2008
