2h7g

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[[Image:2h7g.gif|left|200px]]
[[Image:2h7g.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2h7g |SIZE=350|CAPTION= <scene name='initialview01'>2h7g</scene>, resolution 1.900&Aring;
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The line below this paragraph, containing "STRUCTURE_2h7g", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_topoisomerase DNA topoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.2 5.99.1.2] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= TOP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10255 Variola virus])
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-->
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|DOMAIN=
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{{STRUCTURE_2h7g| PDB=2h7g | SCENE= }}
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|RELATEDENTRY=[[2h7f|2H7F]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h7g OCA], [http://www.ebi.ac.uk/pdbsum/2h7g PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2h7g RCSB]</span>
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}}
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'''Structure of variola topoisomerase non-covalently bound to DNA'''
'''Structure of variola topoisomerase non-covalently bound to DNA'''
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[[Category: Hwang, Y.]]
[[Category: Hwang, Y.]]
[[Category: Perry, K.]]
[[Category: Perry, K.]]
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[[Category: dna binding]]
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[[Category: Dna binding]]
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[[Category: isomerase]]
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[[Category: Isomerase]]
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[[Category: protein-dna complex]]
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[[Category: Protein-dna complex]]
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[[Category: type ib topoisomerase]]
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[[Category: Type ib topoisomerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:57:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:26:33 2008''
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Revision as of 02:57, 4 May 2008

Image:2h7g.gif

Template:STRUCTURE 2h7g

Structure of variola topoisomerase non-covalently bound to DNA


Overview

Although smallpox has been eradicated from the human population, it is presently feared as a possible agent of bioterrorism. The smallpox virus codes for its own topoisomerase enzyme that differs from its cellular counterpart by requiring a specific DNA sequence for activation of catalysis. Here we present crystal structures of the smallpox virus topoisomerase enzyme bound both covalently and noncovalently to a specific DNA sequence. These structures reveal the basis for site-specific DNA recognition, and they explain how catalysis is likely activated by formation of a specific enzyme-DNA interface. Unexpectedly, the poxvirus enzyme uses a major groove binding alpha helix that is not present in the human enzyme to recognize part of the core recognition sequence and activate the enzyme for catalysis. The topoisomerase-DNA complex structures also provide a three-dimensional framework that may facilitate the rational design of therapeutic agents to treat poxvirus infections.

About this Structure

2H7G is a Single protein structure of sequence from Variola virus. Full crystallographic information is available from OCA.

Reference

Structural basis for specificity in the poxvirus topoisomerase., Perry K, Hwang Y, Bushman FD, Van Duyne GD, Mol Cell. 2006 Aug 4;23(3):343-54. PMID:16885024 Page seeded by OCA on Sun May 4 05:57:41 2008

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