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2id8

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[[Image:2id8.gif|left|200px]]
[[Image:2id8.gif|left|200px]]
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{{Structure
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|PDB= 2id8 |SIZE=350|CAPTION= <scene name='initialview01'>2id8</scene>, resolution 1.27&Aring;
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The line below this paragraph, containing "STRUCTURE_2id8", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=2DB:(S)-(2,3-DIHYDROXYPROPOXY)TRIHYDROXYBORATE'>2DB</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Peptidase_K Peptidase K], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.64 3.4.21.64] </span>
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|GENE=
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{{STRUCTURE_2id8| PDB=2id8 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2id8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2id8 OCA], [http://www.ebi.ac.uk/pdbsum/2id8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2id8 RCSB]</span>
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'''Crystal structure of Proteinase K'''
'''Crystal structure of Proteinase K'''
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[[Category: Dauter, Z.]]
[[Category: Dauter, Z.]]
[[Category: Wang, J.]]
[[Category: Wang, J.]]
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[[Category: proteinase]]
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[[Category: Proteinase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:21:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:42:43 2008''
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Revision as of 04:21, 4 May 2008

Image:2id8.gif

Template:STRUCTURE 2id8

Crystal structure of Proteinase K


Overview

X-ray single-wavelength anomalous diffraction (SAD) data from a crystal of proteinase K were collected using synchrotron radiation of 0.98 A wavelength at SER-CAT 22-ID beamline, Advanced Photon Source, Argonne National Laboratory. At this wavelength, the expected Bijvoet ratio resulting from the presence of one calcium, one chloride and ten S atoms in the 279-residue protein is extremely small at approximately 0.46%. The direct-methods program SHELXD located 11 anomalous sites using data truncated to 2 A resolution. SHELXE was used to produce an easily interpretable electron-density map. This study shows that an accurate beamline and a good-quality crystal provide the possibility of successfully using a very weak anomalous signal of sulfur measured at a short wavelength for phasing a protein structure, even if a small degree of radiation damage is present.

About this Structure

2ID8 is a Single protein structure of sequence from Engyodontium album. Full crystallographic information is available from OCA.

Reference

What can be done with a good crystal and an accurate beamline?, Wang J, Dauter M, Dauter Z, Acta Crystallogr D Biol Crystallogr. 2006 Dec;62(Pt 12):1475-83. Epub 2006, Nov 23. PMID:17139083 Page seeded by OCA on Sun May 4 07:21:42 2008

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