2itj
From Proteopedia
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'''Origin binding domain of the SV40 large T antigen (residues 131-259). P212121 crystal form''' | '''Origin binding domain of the SV40 large T antigen (residues 131-259). P212121 crystal form''' | ||
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[[Category: Bochkareva, E.]] | [[Category: Bochkareva, E.]] | ||
[[Category: Martynowski, D.]] | [[Category: Martynowski, D.]] | ||
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| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:51:07 2008'' | |
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Revision as of 04:51, 4 May 2008
Origin binding domain of the SV40 large T antigen (residues 131-259). P212121 crystal form
Overview
The large T antigen (T-ag) protein binds to and activates DNA replication from the origin of DNA replication (ori) in simian virus 40 (SV40). Here, we determined the crystal structures of the T-ag origin-binding domain (OBD) in apo form, and bound to either a 17 bp palindrome (sites 1 and 3) or a 23 bp ori DNA palindrome comprising all four GAGGC binding sites for OBD. The T-ag OBDs were shown to interact with the DNA through a loop comprising Ser147-Thr155 (A1 loop), a combination of a DNA-binding helix and loop (His203-Asn210), and Asn227. The A1 loop traveled back-and-forth along the major groove and accounted for most of the sequence-determining contacts with the DNA. Unexpectedly, in both T-ag-DNA structures, the T-ag OBDs bound DNA independently and did not make direct protein-protein contacts. The T-ag OBD was also captured bound to a non-consensus site ATGGC even in the presence of its canonical site GAGGC. Our observations taken together with the known biochemical and structural features of the T-ag-origin interaction suggest a model for origin unwinding.
About this Structure
2ITJ is a Single protein structure of sequence from Simian virus 40. Full crystallographic information is available from OCA.
Reference
Structure of the origin-binding domain of simian virus 40 large T antigen bound to DNA., Bochkareva E, Martynowski D, Seitova A, Bochkarev A, EMBO J. 2006 Dec 13;25(24):5961-9. Epub 2006 Nov 30. PMID:17139255 Page seeded by OCA on Sun May 4 07:51:07 2008
