This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2jxm
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2jxm.jpg|left|200px]] | [[Image:2jxm.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2jxm", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_2jxm| PDB=2jxm | SCENE= }} | |
| - | | | + | |
| - | + | ||
| - | }} | + | |
'''Ensemble of twenty structures of the Prochlorothrix hollandica plastocyanin- cytochrome f complex''' | '''Ensemble of twenty structures of the Prochlorothrix hollandica plastocyanin- cytochrome f complex''' | ||
| Line 29: | Line 26: | ||
[[Category: Hulsker, R.]] | [[Category: Hulsker, R.]] | ||
[[Category: Ubbink, M.]] | [[Category: Ubbink, M.]] | ||
| - | [[Category: | + | [[Category: Copper]] |
| - | [[Category: | + | [[Category: Electron transport]] |
| - | [[Category: | + | [[Category: Metal-binding]] |
| - | [[Category: | + | [[Category: Transport]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:22:52 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 06:22, 4 May 2008
Ensemble of twenty structures of the Prochlorothrix hollandica plastocyanin- cytochrome f complex
Overview
The nature of transient protein complexes can range from a highly dynamic ensemble of orientations to a single well-defined state. This represents variation in the equilibrium between the encounter and final, functional state. The transient complex between plastocyanin (Pc) and cytochrome f (cytf) of the cyanobacterium Prochlorothrix hollandica was characterized by NMR spectroscopy. Intermolecular pseudocontact shifts and chemical shift perturbations were used as restraints in docking calculations to determine the structure of the wild-type Pc-cytf complex. The orientation of Pc is similar to orientations found in Pc-cytf complexes from other sources. Electrostatics seems to play a modest role in complex formation. A large variability in the ensemble of lowest energy structures indicates a dynamic nature of the complex. Two unusual hydrophobic patch residues in Pc have been mutated to the residues found in other plastocyanins (Y12G/P14L). The binding constants are similar for the complexes of cytf with wild-type Pc and mutant Pc, but the chemical shift perturbations are smaller for the complex with mutant Pc. Docking calculations for the Y12G/P14L Pc-cytf complex did not produce a converged ensemble of structures. Simulations of the dynamics were performed using the observed averaged NMR parameters as input. The results indicate a surprisingly large amplitude of mobility of Y12G/P14L Pc within the complex. It is concluded that the double mutation shifts the complex further from the well-defined toward the encounter state.
About this Structure
2JXM is a Protein complex structure of sequences from Prochlorothrix hollandica. Full crystallographic information is available from OCA.
Reference
Dynamics in the transient complex of plastocyanin-cytochrome f from Prochlorothrix hollandica., Hulsker R, Baranova MV, Bullerjahn GS, Ubbink M, J Am Chem Soc. 2008 Feb 13;130(6):1985-91. Epub 2008 Jan 18. PMID:18201089 Page seeded by OCA on Sun May 4 09:22:52 2008
