2jxm
From Proteopedia
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'''Ensemble of twenty structures of the Prochlorothrix hollandica plastocyanin- cytochrome f complex''' | '''Ensemble of twenty structures of the Prochlorothrix hollandica plastocyanin- cytochrome f complex''' | ||
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[[Category: Hulsker, R.]] | [[Category: Hulsker, R.]] | ||
[[Category: Ubbink, M.]] | [[Category: Ubbink, M.]] | ||
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| - | [[Category: | + | [[Category: Electron transport]] |
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| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:22:52 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 06:22, 4 May 2008
Ensemble of twenty structures of the Prochlorothrix hollandica plastocyanin- cytochrome f complex
Overview
The nature of transient protein complexes can range from a highly dynamic ensemble of orientations to a single well-defined state. This represents variation in the equilibrium between the encounter and final, functional state. The transient complex between plastocyanin (Pc) and cytochrome f (cytf) of the cyanobacterium Prochlorothrix hollandica was characterized by NMR spectroscopy. Intermolecular pseudocontact shifts and chemical shift perturbations were used as restraints in docking calculations to determine the structure of the wild-type Pc-cytf complex. The orientation of Pc is similar to orientations found in Pc-cytf complexes from other sources. Electrostatics seems to play a modest role in complex formation. A large variability in the ensemble of lowest energy structures indicates a dynamic nature of the complex. Two unusual hydrophobic patch residues in Pc have been mutated to the residues found in other plastocyanins (Y12G/P14L). The binding constants are similar for the complexes of cytf with wild-type Pc and mutant Pc, but the chemical shift perturbations are smaller for the complex with mutant Pc. Docking calculations for the Y12G/P14L Pc-cytf complex did not produce a converged ensemble of structures. Simulations of the dynamics were performed using the observed averaged NMR parameters as input. The results indicate a surprisingly large amplitude of mobility of Y12G/P14L Pc within the complex. It is concluded that the double mutation shifts the complex further from the well-defined toward the encounter state.
About this Structure
2JXM is a Protein complex structure of sequences from Prochlorothrix hollandica. Full crystallographic information is available from OCA.
Reference
Dynamics in the transient complex of plastocyanin-cytochrome f from Prochlorothrix hollandica., Hulsker R, Baranova MV, Bullerjahn GS, Ubbink M, J Am Chem Soc. 2008 Feb 13;130(6):1985-91. Epub 2008 Jan 18. PMID:18201089 Page seeded by OCA on Sun May 4 09:22:52 2008
