2o7c

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:2o7c.jpg|left|200px]]
[[Image:2o7c.jpg|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 2o7c |SIZE=350|CAPTION= <scene name='initialview01'>2o7c</scene>, resolution 1.7&Aring;
+
The line below this paragraph, containing "STRUCTURE_2o7c", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=LLP:2-LYSINE(3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHANE)'>LLP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Methionine_gamma-lyase Methionine gamma-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.4.1.11 4.4.1.11] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE=
+
-->
-
|DOMAIN=
+
{{STRUCTURE_2o7c| PDB=2o7c | SCENE= }}
-
|RELATEDENTRY=
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o7c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o7c OCA], [http://www.ebi.ac.uk/pdbsum/2o7c PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2o7c RCSB]</span>
+
-
}}
+
'''Crystal structure of L-methionine-lyase from Pseudomonas'''
'''Crystal structure of L-methionine-lyase from Pseudomonas'''
Line 34: Line 31:
[[Category: Yamashita, M.]]
[[Category: Yamashita, M.]]
[[Category: Yoshioka, T.]]
[[Category: Yoshioka, T.]]
-
[[Category: cancer]]
+
[[Category: Cancer]]
-
[[Category: lyase]]
+
[[Category: Lyase]]
-
[[Category: methionine]]
+
[[Category: Methionine]]
-
[[Category: plp]]
+
[[Category: Plp]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:24:47 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:13:44 2008''
+

Revision as of 07:24, 4 May 2008

Image:2o7c.jpg

Template:STRUCTURE 2o7c

Crystal structure of L-methionine-lyase from Pseudomonas


Overview

l-Methionine gamma-lyase (EC 4.4.1.11, MGL_Pp) from Pseudomonas putida is a multifunctional enzyme, which belongs to the gamma-family of pyridoxal-5'-phosphate (PLP) dependent enzymes. In this report, we demonstrate that the three-dimensional structure of MGL_Pp has been completely solved by the molecular replacement method to an R-factor of 20.4% at 1.8 A resolution. Detailed information of the overall structure of MGL_Pp supplies a clear picture of the substrate- and PLP-binding pockets. Tyr59 and Arg61 of neighbouring subunits, which are strongly conserved in other gamma-family enzymes, contact the phosphate group of PLP. These residues are important as the main anchor within the active site. Lys240, Asp241 and Arg61 of one partner monomer and Tyr114 and Cys116 of the other partner monomer form a hydrogen-bond network in the MGL active site which is specific for MGLs. It is also suggested that electrostatic interactions at the subunit interface are involved in the stabilization of the structural conformation. The detailed structure will facilitate the development of MGL_Pp as an anticancer drug.

About this Structure

2O7C is a Single protein structure of sequence from Pseudomonas putida. Full crystallographic information is available from OCA.

Reference

Structure of the antitumour enzyme L-methionine gamma-lyase from Pseudomonas putida at 1.8 A resolution., Kudou D, Misaki S, Yamashita M, Tamura T, Takakura T, Yoshioka T, Yagi S, Hoffman RM, Takimoto A, Esaki N, Inagaki K, J Biochem. 2007 Apr;141(4):535-44. Epub 2007 Feb 8. PMID:17289792 Page seeded by OCA on Sun May 4 10:24:47 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2o7c"
Personal tools