2ooe
From Proteopedia
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'''Crystal structure of HAT domain of murine CstF-77''' | '''Crystal structure of HAT domain of murine CstF-77''' | ||
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[[Category: Manley, J L.]] | [[Category: Manley, J L.]] | ||
[[Category: Tong, L.]] | [[Category: Tong, L.]] | ||
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Revision as of 08:19, 4 May 2008
Crystal structure of HAT domain of murine CstF-77
Overview
Cleavage stimulation factor (CstF) is a heterotrimeric protein complex essential for polyadenylation of mRNA precursors. The 77 kDa subunit, CstF-77, is known to mediate interactions with the other two subunits of CstF as well as with other components of the polyadenylation machinery. We report here the crystal structure of the HAT (half a TPR) domain of murine CstF-77, as well as its C-terminal subdomain. Structural and biochemical studies show that the HAT domain consists of two subdomains, HAT-N and HAT-C domains, with drastically different orientations of their helical motifs. The structures reveal a highly elongated dimer, spanning 165 A, with the dimerization mediated by the HAT-C domain. Light-scattering studies, yeast two-hybrid assays, and analytical ultracentrifugation measurements confirm this self-association. The mode of dimerization and the relative arrangement of the HAT-N and HAT-C domains are unique to CstF-77. Our data support a role for CstF dimerization in pre-mRNA 3' end processing.
About this Structure
2OOE is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Crystal structure of murine CstF-77: dimeric association and implications for polyadenylation of mRNA precursors., Bai Y, Auperin TC, Chou CY, Chang GG, Manley JL, Tong L, Mol Cell. 2007 Mar 23;25(6):863-75. PMID:17386263 Page seeded by OCA on Sun May 4 11:19:28 2008
