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2ovn
From Proteopedia
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[[Image:2ovn.jpg|left|200px]] | [[Image:2ovn.jpg|left|200px]] | ||
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'''NMR structure of the GCN4 trigger peptide''' | '''NMR structure of the GCN4 trigger peptide''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2OVN is a [[Single protein]] structure | + | 2OVN is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OVN OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Alexandrescu, A T.]] | [[Category: Alexandrescu, A T.]] | ||
[[Category: Matousek, W M.]] | [[Category: Matousek, W M.]] | ||
| - | [[Category: | + | [[Category: Coiled-coil]] |
| - | [[Category: | + | [[Category: Gcn4]] |
| - | [[Category: | + | [[Category: Trigger peptide]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:45:35 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 08:45, 4 May 2008
NMR structure of the GCN4 trigger peptide
Overview
Coiled coils have attracted considerable interest as design templates in a wide range of applications. Successful coiled-coil design strategies therefore require a detailed understanding of coiled-coil folding. One common feature shared by coiled coils is the presence of a short autonomous helical folding unit, termed "trigger sequence," that is indispensable for folding. Detailed knowledge of trigger sequences at the molecular level is thus key to a general understanding of coiled-coil formation. Using a multidisciplinary approach, we identify and characterize here the molecular determinants that specify the helical conformation of the monomeric early folding intermediate of the GCN4 coiled coil. We demonstrate that a network of hydrogen-bonding and electrostatic interactions stabilize the trigger-sequence helix. This network is rearranged in the final dimeric coiled-coil structure, and its destabilization significantly slows down GCN4 leucine zipper folding. Our findings provide a general explanation for the molecular mechanism of coiled-coil formation.
About this Structure
2OVN is a Single protein structure. Full crystallographic information is available from OCA.
Reference
Molecular basis of coiled-coil formation., Steinmetz MO, Jelesarov I, Matousek WM, Honnappa S, Jahnke W, Missimer JH, Frank S, Alexandrescu AT, Kammerer RA, Proc Natl Acad Sci U S A. 2007 Apr 24;104(17):7062-7. Epub 2007 Apr 16. PMID:17438295 Page seeded by OCA on Sun May 4 11:45:35 2008
