2owc

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[[Image:2owc.jpg|left|200px]]
[[Image:2owc.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2owc |SIZE=350|CAPTION= <scene name='initialview01'>2owc</scene>, resolution 2.05&Aring;
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The line below this paragraph, containing "STRUCTURE_2owc", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ACR:ACARBOSE'>ACR</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=SUI:(3-AMINO-2,5-DIOXO-1-PYRROLIDINYL)ACETIC+ACID'>SUI</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/4-alpha-glucanotransferase 4-alpha-glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.25 2.4.1.25] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= malq ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus])
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-->
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|DOMAIN=
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{{STRUCTURE_2owc| PDB=2owc | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2owc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2owc OCA], [http://www.ebi.ac.uk/pdbsum/2owc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2owc RCSB]</span>
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}}
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'''Structure of a covalent intermediate in Thermus thermophilus amylomaltase'''
'''Structure of a covalent intermediate in Thermus thermophilus amylomaltase'''
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[[Category: Kaper, T.]]
[[Category: Kaper, T.]]
[[Category: Maarel, M J.E C.van der.]]
[[Category: Maarel, M J.E C.van der.]]
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[[Category: alpha-amylase]]
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[[Category: Alpha-amylase]]
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[[Category: amylomaltase]]
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[[Category: Amylomaltase]]
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[[Category: beta-alpha-barrel]]
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[[Category: Beta-alpha-barrel]]
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[[Category: glycosyl-enzyme]]
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[[Category: Glycosyl-enzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:47:39 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:24:07 2008''
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Revision as of 08:47, 4 May 2008

Image:2owc.jpg

Template:STRUCTURE 2owc

Structure of a covalent intermediate in Thermus thermophilus amylomaltase


Overview

Amylomaltases are glycosyl hydrolases belonging to glycoside hydrolase family 77 that are capable of the synthesis of large cyclic glucans and the disproportionation of oligosaccharides. Using protein crystallography, we have generated a flip book movie of the amylomaltase catalytic cycle in atomic detail. The structures include a covalent glycosyl enzyme intermediate and a covalent intermediate in complex with an analogue of a co-substrate and show how the structures of both enzyme and substrate respond to the changes required by the catalytic cycle as it proceeds. Notably, the catalytic nucleophile changes conformation dramatically during the reaction. Also, Gln-256 on the 250s loop is involved in orienting the substrate in the +1 site. The absence of a suitable base in the covalent intermediate structure explains the low hydrolysis activity.

About this Structure

2OWC is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

Three-way stabilization of the covalent intermediate in amylomaltase, an alpha-amylase-like transglycosylase., Barends TR, Bultema JB, Kaper T, van der Maarel MJ, Dijkhuizen L, Dijkstra BW, J Biol Chem. 2007 Jun 8;282(23):17242-9. Epub 2007 Apr 9. PMID:17420245 Page seeded by OCA on Sun May 4 11:47:39 2008

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