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2ozo
From Proteopedia
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[[Image:2ozo.jpg|left|200px]] | [[Image:2ozo.jpg|left|200px]] | ||
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'''Autoinhibited intact human ZAP-70''' | '''Autoinhibited intact human ZAP-70''' | ||
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[[Category: Kuriyan, J.]] | [[Category: Kuriyan, J.]] | ||
[[Category: Weiss, A.]] | [[Category: Weiss, A.]] | ||
| - | [[Category: | + | [[Category: Autoinhibition]] |
| - | [[Category: | + | [[Category: Hydrogen bonding network]] |
| - | [[Category: | + | [[Category: Inactive zap-70]] |
| - | [[Category: | + | [[Category: Itam]] |
| - | [[Category: | + | [[Category: Tandem sh2]] |
| - | [[Category: | + | [[Category: Tcr signaling]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 12:00:24 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 09:00, 4 May 2008
Autoinhibited intact human ZAP-70
Overview
ZAP-70, a cytoplasmic tyrosine kinase required for T cell antigen receptor signaling, is controlled by a regulatory segment that includes a tandem SH2 unit responsible for binding to immunoreceptor tyrosine-based activation motifs (ITAMs). The crystal structure of autoinhibited ZAP-70 reveals that the inactive kinase domain adopts a conformation similar to that of cyclin-dependent kinases and Src kinases. The autoinhibitory mechanism of ZAP-70 is, however, distinct and involves interactions between the regulatory segment and the hinge region of the kinase domain that reduce its flexibility. Two tyrosine residues in the SH2-kinase linker that activate ZAP-70 when phosphorylated are involved in aromatic-aromatic interactions that connect the linker to the kinase domain. These interactions are inconsistent with ITAM binding, suggesting that destabilization of this autoinhibited ZAP-70 conformation is the first step in kinase activation.
About this Structure
2OZO is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural basis for the inhibition of tyrosine kinase activity of ZAP-70., Deindl S, Kadlecek TA, Brdicka T, Cao X, Weiss A, Kuriyan J, Cell. 2007 May 18;129(4):735-46. PMID:17512407 Page seeded by OCA on Sun May 4 12:00:24 2008
