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2p1d

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[[Image:2p1d.gif|left|200px]]
[[Image:2p1d.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2p1d |SIZE=350|CAPTION= <scene name='initialview01'>2p1d</scene>, resolution 2.90&Aring;
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The line below this paragraph, containing "STRUCTURE_2p1d", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=5GP:GUANOSINE-5&#39;-MONOPHOSPHATE'>5GP</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= NSP5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11060 Dengue virus 2])
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-->
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|DOMAIN=
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{{STRUCTURE_2p1d| PDB=2p1d | SCENE= }}
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|RELATEDENTRY=[[1l9k|1L9K]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2p1d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p1d OCA], [http://www.ebi.ac.uk/pdbsum/2p1d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2p1d RCSB]</span>
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}}
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'''Crystal structure of dengue methyltransferase in complex with GTP and S-Adenosyl-L-homocysteine'''
'''Crystal structure of dengue methyltransferase in complex with GTP and S-Adenosyl-L-homocysteine'''
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[[Category: Egloff, M P.]]
[[Category: Egloff, M P.]]
[[Category: MSGP, Marseilles Structural Genomics Program.@.AFMB.]]
[[Category: MSGP, Marseilles Structural Genomics Program.@.AFMB.]]
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[[Category: dengue virus methyltransferase]]
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[[Category: Dengue virus methyltransferase]]
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[[Category: marseilles structural genomics program @ afmb]]
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[[Category: Marseilles structural genomics program @ afmb]]
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[[Category: msgp]]
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[[Category: Msgp]]
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[[Category: structural genomic]]
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[[Category: Structural genomic]]
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[[Category: viral enzymes involved in replication]]
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[[Category: Viral enzymes involved in replication]]
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[[Category: vizier]]
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[[Category: Vizier]]
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[[Category: vizier. viral enzymes involved in replication]]
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[[Category: Vizier. viral enzymes involved in replication]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 12:08:01 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:26:49 2008''
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Revision as of 09:08, 4 May 2008

Image:2p1d.gif

Template:STRUCTURE 2p1d

Crystal structure of dengue methyltransferase in complex with GTP and S-Adenosyl-L-homocysteine


Overview

Viruses represent an attractive system with which to study the molecular basis of mRNA capping and its relation to the RNA transcription machinery. The RNA-dependent RNA polymerase NS5 of flaviviruses presents a characteristic motif of S-adenosyl-L-methionine-dependent methyltransferases at its N-terminus, and polymerase motifs at its C-terminus. The crystal structure of an N-terminal fragment of Dengue virus type 2 NS5 is reported at 2.4 A resolution. We show that this NS5 domain includes a typical methyltransferase core and exhibits a (nucleoside-2'-O-)-methyltransferase activity on capped RNA. The structure of a ternary complex comprising S-adenosyl-L-homocysteine and a guanosine triphosphate (GTP) analogue shows that 54 amino acids N-terminal to the core provide a novel GTP-binding site that selects guanine using a previously unreported mechanism. Binding studies using GTP- and RNA cap-analogues, as well as the spatial arrangement of the methyltransferase active site relative to the GTP-binding site, suggest that the latter is a specific cap-binding site. As RNA capping is an essential viral function, these results provide a structural basis for the rational design of drugs against the emerging flaviviruses.

About this Structure

2P1D is a Single protein structure of sequence from Dengue virus 2. Full crystallographic information is available from OCA.

Reference

An RNA cap (nucleoside-2'-O-)-methyltransferase in the flavivirus RNA polymerase NS5: crystal structure and functional characterization., Egloff MP, Benarroch D, Selisko B, Romette JL, Canard B, EMBO J. 2002 Jun 3;21(11):2757-68. PMID:12032088 Page seeded by OCA on Sun May 4 12:08:01 2008

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