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2pwt
From Proteopedia
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'''Crystal structure of the bacterial ribosomal decoding site complexed with aminoglycoside containing the L-HABA group''' | '''Crystal structure of the bacterial ribosomal decoding site complexed with aminoglycoside containing the L-HABA group''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PWT OCA]. | |
==Reference== | ==Reference== | ||
Crystal Structure of the Bacterial Ribosomal Decoding Site Complexed with a Synthetic Doubly Functionalized Paromomycin Derivative: a New Specific Binding Mode to an A-Minor Motif Enhances in vitro Antibacterial Activity., Kondo J, Pachamuthu K, Francois B, Szychowski J, Hanessian S, Westhof E, ChemMedChem. 2007 Nov 12;2(11):1631-1638. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17722211 17722211] | Crystal Structure of the Bacterial Ribosomal Decoding Site Complexed with a Synthetic Doubly Functionalized Paromomycin Derivative: a New Specific Binding Mode to an A-Minor Motif Enhances in vitro Antibacterial Activity., Kondo J, Pachamuthu K, Francois B, Szychowski J, Hanessian S, Westhof E, ChemMedChem. 2007 Nov 12;2(11):1631-1638. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17722211 17722211] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Francois, B.]] | [[Category: Francois, B.]] | ||
[[Category: Hanessian, S.]] | [[Category: Hanessian, S.]] | ||
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[[Category: Szychowski, J.]] | [[Category: Szychowski, J.]] | ||
[[Category: Westhof, E.]] | [[Category: Westhof, E.]] | ||
| - | [[Category: | + | [[Category: Aminoglycoside]] |
| - | [[Category: | + | [[Category: Haba group]] |
| - | [[Category: | + | [[Category: Ribosomal decoding site]] |
| - | [[Category: | + | [[Category: Rna]] |
| - | [[Category: | + | [[Category: X-ray analysis]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:55:37 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 10:55, 4 May 2008
Crystal structure of the bacterial ribosomal decoding site complexed with aminoglycoside containing the L-HABA group
Overview
The crystal structure of the complex between oligonucleotide containing the bacterial ribosomal decoding site (A site) and the synthetic paromomycin analogue 1, which contains the gamma-amino-alpha-hydroxybutyryl (L-haba) group at position N1 of ring II (2-DOS ring), and an ether chain with an O-phenethylaminoethyl group at position C2 of ring III, is reported. Interestingly, next to the paromomycin analogue 1 specifically bound to the A site, a second molecule of 1 with a different conformation is observed at the crystal packing interface which mimics the A-minor interaction between two bulged-out adenines from the A site and the codon-anticodon stem of the mRNA-tRNA complex. Improved antibacterial activity supports the conclusion that analogue 1 might affect protein synthesis on the ribosome in two different ways: 1) specific binding to the A site forces maintenance of the "on" state with two bulged out adenines, and 2) a new binding mode of 1 to an A-minor motif which stabilizes complex formation between the ribosome and the mRNA-tRNA complex regardless of whether the codon-anticodon stem is of the cognate or near-cognate type.
About this Structure
Full crystallographic information is available from OCA.
Reference
Crystal Structure of the Bacterial Ribosomal Decoding Site Complexed with a Synthetic Doubly Functionalized Paromomycin Derivative: a New Specific Binding Mode to an A-Minor Motif Enhances in vitro Antibacterial Activity., Kondo J, Pachamuthu K, Francois B, Szychowski J, Hanessian S, Westhof E, ChemMedChem. 2007 Nov 12;2(11):1631-1638. PMID:17722211 Page seeded by OCA on Sun May 4 13:55:37 2008
