2q8h
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2q8h.gif|left|200px]] | [[Image:2q8h.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2q8h", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | | | + | --> |
| - | | | + | {{STRUCTURE_2q8h| PDB=2q8h | SCENE= }} |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Structure of pyruvate dehydrogenase kinase isoform 1 in complex with dichloroacetate (DCA)''' | '''Structure of pyruvate dehydrogenase kinase isoform 1 in complex with dichloroacetate (DCA)''' | ||
| Line 25: | Line 22: | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: [Pyruvate dehydrogenase (acetyl-transferring)] kinase]] | ||
[[Category: Chuang, D T.]] | [[Category: Chuang, D T.]] | ||
[[Category: Chuang, J L.]] | [[Category: Chuang, J L.]] | ||
[[Category: Kato, M.]] | [[Category: Kato, M.]] | ||
[[Category: Li, J.]] | [[Category: Li, J.]] | ||
| - | [[Category: | + | [[Category: Dichroloacetate]] |
| - | [[Category: | + | [[Category: Ghkl atpase/kinase family]] |
| - | [[Category: | + | [[Category: Mitochondrial kinase]] |
| - | [[Category: | + | [[Category: Pyruvate dehydrogenase complex]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:32:02 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 11:32, 4 May 2008
Structure of pyruvate dehydrogenase kinase isoform 1 in complex with dichloroacetate (DCA)
Overview
Pyruvate dehydrogenase kinase (PDK) isoforms are molecular switches that downregulate the pyruvate dehydrogenase complex (PDC) by reversible phosphorylation in mitochondria. We have determined structures of human PDK1 or PDK3 bound to the inhibitors AZD7545, dichloroacetate (DCA), and radicicol. We show that the trifluoromethylpropanamide end of AZD7545 projects into the lipoyl-binding pocket of PDK1. This interaction results in inhibition of PDK1 and PDK3 activities by aborting kinase binding to the PDC scaffold. Paradoxically, AZD7545 at saturating concentrations robustly increases scaffold-free PDK3 activity, similar to the inner lipoyl domain. Good DCA density is present in the helix bundle in the N-terminal domain of PDK1. Bound DCA promotes local conformational changes that are communicated to both nucleotide-binding and lipoyl-binding pockets of PDK1, leading to the inactivation of kinase activity. Finally, radicicol inhibits kinase activity by binding directly to the ATP-binding pocket of PDK3, similar to Hsp90 and Topo VI from the same ATPase/kinase superfamily.
About this Structure
2Q8H is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Distinct structural mechanisms for inhibition of pyruvate dehydrogenase kinase isoforms by AZD7545, dichloroacetate, and radicicol., Kato M, Li J, Chuang JL, Chuang DT, Structure. 2007 Aug;15(8):992-1004. Epub 2007 Aug 2. PMID:17683942 Page seeded by OCA on Sun May 4 14:32:02 2008
