3bep

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[[Image:3bep.jpg|left|200px]]
[[Image:3bep.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 3bep |SIZE=350|CAPTION= <scene name='initialview01'>3bep</scene>, resolution 1.920&Aring;
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The line below this paragraph, containing "STRUCTURE_3bep", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:5cy+Binding+Site+For+Residue+C+111'>AC1</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=5CY:N,N&#39;-(DIPROPYL)-TETRAMETHYLINDODICARBOCYANINE'>5CY</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= dnaN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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-->
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|DOMAIN=
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{{STRUCTURE_3bep| PDB=3bep | SCENE= }}
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|RELATEDENTRY=[[2pol|2POL]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bep FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bep OCA], [http://www.ebi.ac.uk/pdbsum/3bep PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3bep RCSB]</span>
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}}
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'''Structure of a sliding clamp on DNA'''
'''Structure of a sliding clamp on DNA'''
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[[Category: Kuriyan, J.]]
[[Category: Kuriyan, J.]]
[[Category: Yurieva, O.]]
[[Category: Yurieva, O.]]
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[[Category: beta subunit]]
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[[Category: Beta subunit]]
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[[Category: dna complex]]
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[[Category: Dna complex]]
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[[Category: e. coli polymerase iii]]
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[[Category: E. coli polymerase iii]]
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[[Category: sliding clamp]]
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[[Category: Sliding clamp]]
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[[Category: transcription/dna complex]]
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[[Category: Transcription/dna complex]]
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[[Category: transferase]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:41:33 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:25:34 2008''
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Revision as of 17:41, 4 May 2008

Image:3bep.jpg

Template:STRUCTURE 3bep

Structure of a sliding clamp on DNA


Overview

The structure of the E. coli beta clamp polymerase processivity factor has been solved in complex with primed DNA. Interestingly, the clamp directly binds the DNA duplex and also forms a crystal contact with the ssDNA template strand, which binds into the protein-binding pocket of the clamp. We demonstrate that these clamp-DNA interactions function in clamp loading, perhaps by inducing the ring to close around DNA. Clamp binding to template ssDNA may also serve to hold the clamp at a primed site after loading or during switching of multiple factors on the clamp. Remarkably, the DNA is highly tilted as it passes through the beta ring. The pronounced 22 degrees angle of DNA through beta may enable DNA to switch between multiple factors bound to a single clamp simply by alternating from one protomer of the ring to the other.

About this Structure

3BEP is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structure of a sliding clamp on DNA., Georgescu RE, Kim SS, Yurieva O, Kuriyan J, Kong XP, O'Donnell M, Cell. 2008 Jan 11;132(1):43-54. PMID:18191219 Page seeded by OCA on Sun May 4 20:41:33 2008

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OCA

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