3c17

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(New page: 200px {{Structure |PDB= 3c17 |SIZE=350|CAPTION= <scene name='initialview01'>3c17</scene>, resolution 1.95&Aring; |SITE= <scene name='pdbsite=AC1:Na+Binding+Site+F...)
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[[Image:3c17.jpg|left|200px]]
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{{Structure
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|PDB= 3c17 |SIZE=350|CAPTION= <scene name='initialview01'>3c17</scene>, resolution 1.95&Aring;
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The line below this paragraph, containing "STRUCTURE_3c17", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Na+Binding+Site+For+Residue+A+1'>AC1</scene>, <scene name='pdbsite=AC2:Na+Binding+Site+For+Residue+B+322'>AC2</scene>, <scene name='pdbsite=AC3:Na+Binding+Site+For+Residue+A+322'>AC3</scene>, <scene name='pdbsite=AC4:Na+Binding+Site+For+Residue+B+323'>AC4</scene>, <scene name='pdbsite=AC5:Na+Binding+Site+For+Residue+A+323'>AC5</scene>, <scene name='pdbsite=AC6:Na+Binding+Site+For+Residue+B+324'>AC6</scene>, <scene name='pdbsite=AC7:Na+Binding+Site+For+Residue+A+324'>AC7</scene>, <scene name='pdbsite=AC8:Na+Binding+Site+For+Residue+B+325'>AC8</scene>, <scene name='pdbsite=AC9:Na+Binding+Site+For+Residue+B+326'>AC9</scene>, <scene name='pdbsite=BC1:Na+Binding+Site+For+Residue+B+327'>BC1</scene>, <scene name='pdbsite=BC2:Na+Binding+Site+For+Residue+A+325'>BC2</scene>, <scene name='pdbsite=BC3:Cl+Binding+Site+For+Residue+A+326'>BC3</scene>, <scene name='pdbsite=BC4:Cl+Binding+Site+For+Residue+B+328'>BC4</scene>, <scene name='pdbsite=BC5:Cl+Binding+Site+For+Residue+A+327'>BC5</scene>, <scene name='pdbsite=BC6:Cl+Binding+Site+For+Residue+B+329'>BC6</scene>, <scene name='pdbsite=BC7:Cl+Binding+Site+For+Residue+B+330'>BC7</scene>, <scene name='pdbsite=BC8:Cl+Binding+Site+For+Residue+A+328'>BC8</scene>, <scene name='pdbsite=BC9:Cl+Binding+Site+For+Residue+B+331'>BC9</scene>, <scene name='pdbsite=CC1:Cl+Binding+Site+For+Residue+A+329'>CC1</scene>, <scene name='pdbsite=CC2:Cl+Binding+Site+For+Residue+A+330'>CC2</scene>, <scene name='pdbsite=CC3:Cl+Binding+Site+For+Residue+B+332'>CC3</scene>, <scene name='pdbsite=CC4:Cl+Binding+Site+For+Residue+A+331'>CC4</scene>, <scene name='pdbsite=CC5:Cl+Binding+Site+For+Residue+B+333'>CC5</scene>, <scene name='pdbsite=CC6:Cl+Binding+Site+For+Residue+B+334'>CC6</scene>, <scene name='pdbsite=CC7:Cl+Binding+Site+For+Residue+A+332'>CC7</scene>, <scene name='pdbsite=CC8:Cl+Binding+Site+For+Residue+B+335'>CC8</scene>, <scene name='pdbsite=CC9:Cl+Binding+Site+For+Residue+A+333'>CC9</scene> and <scene name='pdbsite=DC1:Cl+Binding+Site+For+Residue+A+334'>DC1</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
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|GENE= ybiK (iaaA) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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-->
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd04701 Asparaginase_2]</span>
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{{STRUCTURE_3c17| PDB=3c17 | SCENE= }}
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|RELATEDENTRY=[[2zak|2ZAK]], [[1k2x|1K2X]], [[1jn9|1JN9]], [[2zal|2ZAL]], [[1t3m|1T3M]], [[2gez|2GEZ]], [[9gac|9GAC]], [[9gaf|9GAF]], [[9gaa|9GAA]], [[2gac|2GAC]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c17 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c17 OCA], [http://www.ebi.ac.uk/pdbsum/3c17 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3c17 RCSB]</span>
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}}
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'''Hexagonal Crystal Structure of Precursor E. coli Isoaspartyl Peptidase/l-Asparaginase (ECAIII) with Active-site T179A mutation'''
'''Hexagonal Crystal Structure of Precursor E. coli Isoaspartyl Peptidase/l-Asparaginase (ECAIII) with Active-site T179A mutation'''
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[[Category: Jaskolski, M.]]
[[Category: Jaskolski, M.]]
[[Category: Michalska, K.]]
[[Category: Michalska, K.]]
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[[Category: asparaginase]]
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[[Category: Asparaginase]]
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[[Category: autoproteolysis]]
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[[Category: Autoproteolysis]]
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[[Category: isoaspartyl peptidase]]
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[[Category: Isoaspartyl peptidase]]
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[[Category: ntn-hydrolase]]
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[[Category: Ntn-hydrolase]]
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[[Category: precursor]]
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[[Category: Precursor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 21:16:36 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 2 11:59:51 2008''
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Revision as of 18:16, 4 May 2008

Image:3c17.jpg

Template:STRUCTURE 3c17

Hexagonal Crystal Structure of Precursor E. coli Isoaspartyl Peptidase/l-Asparaginase (ECAIII) with Active-site T179A mutation


Overview

Plant L-asparaginases and their bacterial homologs, such as EcAIII found in E. coli, form a subgroup of the Ntn-hydrolase family. In common with all Ntn-hydrolases, they are expressed as inactive precursors that undergo activation in an autocatalytic manner. The maturation process involves intramolecular hydrolysis of a single peptide bond, leading to the formation of two subunits (alpha and beta) folded as one structural domain, with the nucleophilic Thr residue located at the freed N-terminus of subunit beta. The mechanism of the autocleavage reaction remains obscure. We have determined the crystal structure of an active-site mutant of EcAIII, with the catalytic Thr residue substituted by Ala (T179A). The modification has led to a correctly folded but unprocessed molecule, revealing the geometry and molecular environment of the scissile peptide bond. The autocatalytic reaction is analyzed from the point of view of the Thr179 side chain rotation, identification of a potential general-base residue, and the architecture of the oxyanion hole.

About this Structure

3C17 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

The mechanism of autocatalytic activation of plant-type L-asparaginases., Michalska K, Hernandez-Santoyo A, Jaskolski M, J Biol Chem. 2008 Mar 10;. PMID:18334484 Page seeded by OCA on Sun May 4 21:16:36 2008

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