3lyo

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[[Image:3lyo.jpg|left|200px]]
[[Image:3lyo.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 3lyo |SIZE=350|CAPTION= <scene name='initialview01'>3lyo</scene>, resolution 1.93&Aring;
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The line below this paragraph, containing "STRUCTURE_3lyo", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CCN:ACETONITRILE'>CCN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_3lyo| PDB=3lyo | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3lyo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lyo OCA], [http://www.ebi.ac.uk/pdbsum/3lyo PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3lyo RCSB]</span>
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}}
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'''CROSS-LINKED CHICKEN LYSOZYME CRYSTAL IN 95% ACETONITRILE-WATER'''
'''CROSS-LINKED CHICKEN LYSOZYME CRYSTAL IN 95% ACETONITRILE-WATER'''
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[[Category: Wang, Z.]]
[[Category: Wang, Z.]]
[[Category: Zhu, G.]]
[[Category: Zhu, G.]]
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[[Category: cross-linked]]
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[[Category: Cross-linked]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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[[Category: hydrolase (o-glycosyl)]]
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[[Category: Lysozyme]]
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[[Category: lysozyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:06:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:34:27 2008''
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Revision as of 19:06, 4 May 2008

Image:3lyo.jpg

Template:STRUCTURE 3lyo

CROSS-LINKED CHICKEN LYSOZYME CRYSTAL IN 95% ACETONITRILE-WATER


Overview

Tetragonal crystals of hen egg white lysozyme were cross-linked and subjected to X-ray diffraction study in acetonitrile-water media with different acetonitrile concentrations. Crystals in neat acetonitrile did not scatter X-ray well. Structures of crystals in neat water, in 90% and 95% acetonitrile, and crystal back-soaked from acetonitrile to water, were determined to about 2 A resolution. For crystals in both 90% acetonitrile, and crystal back-soaked from acetonitrile to water, were determined to about 2 A resolution. For crystals in both 90% and 95% acetonitrile, only one protein-bond acetonitrile molecule is found in the active site cleft, and its location and binding-protein mode is similar to the C subunit of polysaccharide. The alteration in conformation and hydrogen-bond pattern involving water as solvent causes the reduction of the protein's flexibility in organic media. The back-soaked crystal regained its ordinary three-dimensional structure in water.

About this Structure

3LYO is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.

Reference

X-ray studies on cross-linked lysozyme crystals in acetonitrile-water mixture., Wang Z, Zhu G, Huang Q, Qian M, Shao M, Jia Y, Tang Y, Biochim Biophys Acta. 1998 May 19;1384(2):335-44. PMID:9659395 Page seeded by OCA on Sun May 4 22:06:22 2008

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