3c1v
From Proteopedia
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'''The 1.5 A Crystal structure of Ca2+-bound S100A4''' | '''The 1.5 A Crystal structure of Ca2+-bound S100A4''' | ||
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| + | ==Overview== | ||
| + | S100A4 takes part in control of tumour cell migration and contributes to metastatic spread in in vivo models. In the active dimeric Ca(2+)-bound state it interacts with multiple intracellular targets. Conversely, oligomeric forms of S100A4 are linked with the extracellular function of this protein. We report the 1.5A X-ray crystal structure of Ca(2+)-bound S100A4 and use it to identify the residues involved in target recognition and to derive a model of the oligomeric state. We applied stopped-flow analysis of tyrosine fluorescence to derive kinetics of S100A4 activation by Ca(2+) (k(on)=3.5muM(-1)s(-1), k(off)=20s(-1)). | ||
==About this Structure== | ==About this Structure== | ||
3C1V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C1V OCA]. | 3C1V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C1V OCA]. | ||
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| + | ==Reference== | ||
| + | Crystal structure of the Ca(2+)-form and Ca(2+)-binding kinetics of metastasis-associated protein, S100A4., Gingras AR, Basran J, Prescott A, Kriajevska M, Bagshaw CR, Barsukov IL, FEBS Lett. 2008 May 28;582(12):1651-6. Epub 2008 Apr 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18435928 18435928] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Calcium-bound]] | [[Category: Calcium-bound]] | ||
[[Category: S100a4]] | [[Category: S100a4]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 4 10:01:51 2008'' |
Revision as of 07:01, 4 June 2008
The 1.5 A Crystal structure of Ca2+-bound S100A4
Overview
S100A4 takes part in control of tumour cell migration and contributes to metastatic spread in in vivo models. In the active dimeric Ca(2+)-bound state it interacts with multiple intracellular targets. Conversely, oligomeric forms of S100A4 are linked with the extracellular function of this protein. We report the 1.5A X-ray crystal structure of Ca(2+)-bound S100A4 and use it to identify the residues involved in target recognition and to derive a model of the oligomeric state. We applied stopped-flow analysis of tyrosine fluorescence to derive kinetics of S100A4 activation by Ca(2+) (k(on)=3.5muM(-1)s(-1), k(off)=20s(-1)).
About this Structure
3C1V is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of the Ca(2+)-form and Ca(2+)-binding kinetics of metastasis-associated protein, S100A4., Gingras AR, Basran J, Prescott A, Kriajevska M, Bagshaw CR, Barsukov IL, FEBS Lett. 2008 May 28;582(12):1651-6. Epub 2008 Apr 22. PMID:18435928 Page seeded by OCA on Wed Jun 4 10:01:51 2008
