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2eue

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Revision as of 12:05, 25 June 2008

Template:STRUCTURE 2eue

Crystal structure of the protein kinase domain of yeast AMP-activated protein kinase Snf1


Overview

AMP-activated protein kinase (AMPK) is a master metabolic regulator, and is an important target for drug development against diabetes, obesity, and other diseases. AMPK is a hetero-trimeric enzyme, with a catalytic (alpha) subunit, and two regulatory (beta and gamma) subunits. Here we report the crystal structure at 2.2A resolution of the protein kinase domain (KD) of the catalytic subunit of yeast AMPK (commonly known as SNF1). The Snf1-KD structure shares strong similarity to other protein kinases, with a small N-terminal lobe and a large C-terminal lobe. Two negative surface patches in the structure may be important for the recognition of the substrates of this kinase.

About this Structure

2EUE is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Crystal structure of the protein kinase domain of yeast AMP-activated protein kinase Snf1., Rudolph MJ, Amodeo GA, Bai Y, Tong L, Biochem Biophys Res Commun. 2005 Dec 2;337(4):1224-8. Epub 2005 Oct 7. PMID:16236260

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