1efa

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[[Image:1efa.gif|left|200px]]
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[[Image:1efa.png|left|200px]]
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==Overview==
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{{ABSTRACT_10700279}}
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Crystal structures of the Lac repressor, with and without isopropyithiogalactoside (IPTG), and the repressor bound to operator have provided a model for how the binding of the inducer reduces the affinity of the repressor for the operator. However, because of the low resolution of the operator-bound structure (4.8 A), the model for the allosteric transition was presented in terms of structural elements rather than in terms of side chain interactions. Here we have constructed a dimeric Lac repressor and determined its structure at 2.6 A resolution in complex with a symmetric operator and the anti-inducer orthonitrophenylfucoside (ONPF). The structure enables the induced (IPTG-bound) and repressed (operator-bound) conformations of the repressor to be compared in atomic detail. An extensive network of interactions between the DNA-binding and core domains of the repressor suggests a possible mechanism for the allosteric transition.
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==About this Structure==
==About this Structure==
1EFA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The following page contains interesting information on the relation of 1EFA with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb39_1.html lac Repressor]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EFA OCA].
1EFA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The following page contains interesting information on the relation of 1EFA with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb39_1.html lac Repressor]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EFA OCA].
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==Reference==
 
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A closer view of the conformation of the Lac repressor bound to operator., Bell CE, Lewis M, Nat Struct Biol. 2000 Mar;7(3):209-14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10700279 10700279]
 
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Molecular switch]]
[[Category: Molecular switch]]
[[Category: Protein-dna complex]]
[[Category: Protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:01:45 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 17:13:20 2008''

Revision as of 14:13, 26 June 2008

Image:1efa.png

Template:STRUCTURE 1efa

CRYSTAL STRUCTURE OF THE LAC REPRESSOR DIMER BOUND TO OPERATOR AND THE ANTI-INDUCER ONPF


Template:ABSTRACT 10700279

About this Structure

1EFA is a Single protein structure of sequence from Escherichia coli. The following page contains interesting information on the relation of 1EFA with [lac Repressor]. Full crystallographic information is available from OCA.

Page seeded by OCA on Thu Jun 26 17:13:20 2008

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