2f8s

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[[Image:2f8s.gif|left|200px]]
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[[Image:2f8s.png|left|200px]]
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==Overview==
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{{ABSTRACT_17027504}}
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Argonaute proteins are key components of the RNA-induced silencing complex (RISC). They provide both architectural and catalytic functionalities associated with small interfering RNA (siRNA) guide strand recognition and subsequent guide strand-mediated cleavage of complementary mRNAs. We report on the 3.0 A crystal structures of 22-mer and 26-mer siRNAs bound to Aquifex aeolicus Argonaute (Aa-Ago), where one 2 nt 3' overhang of the siRNA inserts into a cavity positioned on the outer surface of the PAZ-containing lobe of the bilobal Aa-Ago architecture. The first overhang nucleotide stacks over a tyrosine ring, while the second overhang nucleotide, together with the intervening sugar-phosphate backbone, inserts into a preformed surface cavity. Photochemical crosslinking studies on Aa-Ago with 5-iodoU-labeled single-stranded siRNA and siRNA duplex provide support for this externally bound siRNA-Aa-Ago complex. The structure and biochemical data together provide insights into a protein-RNA recognition event potentially associated with the RISC-loading pathway.
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==About this Structure==
==About this Structure==
2F8S is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8S OCA].
2F8S is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8S OCA].
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==Reference==
 
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A potential protein-RNA recognition event along the RISC-loading pathway from the structure of A. aeolicus Argonaute with externally bound siRNA., Yuan YR, Pei Y, Chen HY, Tuschl T, Patel DJ, Structure. 2006 Oct;14(10):1557-65. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17027504 17027504]
 
[[Category: Aquifex aeolicus]]
[[Category: Aquifex aeolicus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Risc loading complex]]
[[Category: Risc loading complex]]
[[Category: Sirna]]
[[Category: Sirna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:35:58 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 18:16:03 2008''

Revision as of 15:16, 26 June 2008

Template:STRUCTURE 2f8s

Crystal structure of Aa-Ago with externally-bound siRNA


Template:ABSTRACT 17027504

About this Structure

2F8S is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.

Page seeded by OCA on Thu Jun 26 18:16:03 2008

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