From Proteopedia
(Difference between revisions)
proteopedia linkproteopedia link
|
|
| Line 1: |
Line 1: |
| - | [[Image:1a04.gif|left|200px]] | + | {{Seed}} |
| | + | [[Image:1a04.png|left|200px]] |
| | | | |
| | <!-- | | <!-- |
| Line 9: |
Line 10: |
| | {{STRUCTURE_1a04| PDB=1a04 | SCENE= }} | | {{STRUCTURE_1a04| PDB=1a04 | SCENE= }} |
| | | | |
| - | '''THE STRUCTURE OF THE NITRATE/NITRITE RESPONSE REGULATOR PROTEIN NARL IN THE MONOCLINIC C2 CRYSTAL FORM'''
| + | ===THE STRUCTURE OF THE NITRATE/NITRITE RESPONSE REGULATOR PROTEIN NARL IN THE MONOCLINIC C2 CRYSTAL FORM=== |
| | | | |
| | | | |
| - | ==Overview==
| + | <!-- |
| - | The structure of the Escherichia coli response regulator NarL has been solved in a new, monoclinic space group, and compared with the earlier orthorhombic crystal structure. Because the monoclinic crystal has two independent NarL molecules per asymmetric unit, we now have three completely independent snapshots of the NarL molecule: two from the monoclinic form and one from the orthorhombic. Comparison of these three structures shows the following: (a) The pairing of N and C domains of the NarL molecule proposed from the earlier analysis is in fact correct, although the polypeptide chain connecting domains was, and remains, disordered and not completely visible. The new structure exhibits identical relative orientation of N and C domains, and supplies some of the missing residues, leaving a gap of only seven amino acids. (b) Examination of corresponding features in the three independent NarL molecules shows that deformations in structure produced by crystal packing are negligible. (c) The "telephone receiver" model of NarL activation is confirmed. The N domain of NarL blocks the binding of DNA to the C domain that would be expected from the helix-turn-helix structure of the C domain. Hence, binding can only occur after significant displacement of N and C domains. (d) NarL monomers have a strong tendency toward dimerization involving contacts between helixes alpha 1 in the two monomers, and this may have mechanistic significance in DNA binding. Analogous involvement of helix alpha 1 in intermolecular contacts is also found in UhpA and in the CheY/CheZ complex. | + | The line below this paragraph, {{ABSTRACT_PUBMED_9521685}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 9521685 is the PubMed ID number. |
| | + | --> |
| | + | {{ABSTRACT_PUBMED_9521685}} |
| | | | |
| | ==About this Structure== | | ==About this Structure== |
| Line 31: |
Line 35: |
| | [[Category: Signal transduction protein]] | | [[Category: Signal transduction protein]] |
| | [[Category: Two-component system]] | | [[Category: Two-component system]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 09:36:32 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 15:42:02 2008'' |
Revision as of 12:42, 30 June 2008
Template:STRUCTURE 1a04
THE STRUCTURE OF THE NITRATE/NITRITE RESPONSE REGULATOR PROTEIN NARL IN THE MONOCLINIC C2 CRYSTAL FORM
Template:ABSTRACT PUBMED 9521685
About this Structure
1A04 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
NarL dimerization? Suggestive evidence from a new crystal form., Baikalov I, Schroder I, Kaczor-Grzeskowiak M, Cascio D, Gunsalus RP, Dickerson RE, Biochemistry. 1998 Mar 17;37(11):3665-76. PMID:9521685
Page seeded by OCA on Mon Jun 30 15:42:02 2008
