1a6j

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[[Image:1a6j.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1a6j| PDB=1a6j | SCENE= }}
{{STRUCTURE_1a6j| PDB=1a6j | SCENE= }}
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'''NITROGEN REGULATORY BACTERIAL PROTEIN IIA-NITROGEN'''
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===NITROGEN REGULATORY BACTERIAL PROTEIN IIA-NITROGEN===
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==Overview==
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The bacterial rpoN operon codes for sigma 54, which is the key sigma factor that, under nitrogen starvation conditions, activates the transcription of genes needed to assimilate ammonia and glutamate. The rpoN operon contains several other open reading frames that are cotranscribed with sigma 54. The product of one of these, the 17.9 kDa protein IIANtr, is homologous to IIA proteins of the phosphoenolpyruvate:sugar phosphotransferase (PTS) system. IIANtr influences the transcription of sigma 54-dependent genes through an unknown mechanism and may thereby provide a regulatory link between carbon and nitrogen metabolism. Here we describe the 2.35 A X-ray structure of Escherichia coli IIANtr. It is the first structure of a IIA enzyme from the fructose-mannitol family of the PTS. The enzyme displays a novel fold characterized by a central mixed parallel/anti-parallel beta-sheet surrounded by six alpha-helices. The active site His73 is situated in a shallow depression on the protein surface.
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(as it appears on PubMed at http://www.pubmed.gov), where 9636714 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9636714}}
==About this Structure==
==About this Structure==
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[[Category: Nitrogen regulation]]
[[Category: Nitrogen regulation]]
[[Category: Phosphotransferase system]]
[[Category: Phosphotransferase system]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 09:53:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 16:14:54 2008''

Revision as of 13:14, 30 June 2008

Image:1a6j.png

Template:STRUCTURE 1a6j

NITROGEN REGULATORY BACTERIAL PROTEIN IIA-NITROGEN

Template:ABSTRACT PUBMED 9636714

About this Structure

1A6J is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

The three-dimensional structure of the nitrogen regulatory protein IIANtr from Escherichia coli., Bordo D, van Monfort RL, Pijning T, Kalk KH, Reizer J, Saier MH Jr, Dijkstra BW, J Mol Biol. 1998 May 29;279(1):245-55. PMID:9636714

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