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1ac3

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[[Image:1ac3.gif|left|200px]]
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{{STRUCTURE_1ac3| PDB=1ac3 | SCENE= }}
{{STRUCTURE_1ac3| PDB=1ac3 | SCENE= }}
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'''SOLUTION STRUCTURE OF AN RNA-DNA HYBRID DUPLEX CONTAINING A 3'-THIOFORMACETAL LINKER AND AN RNA A-TRACT, NMR, 8 STRUCTURES'''
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===SOLUTION STRUCTURE OF AN RNA-DNA HYBRID DUPLEX CONTAINING A 3'-THIOFORMACETAL LINKER AND AN RNA A-TRACT, NMR, 8 STRUCTURES===
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==Overview==
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Neutral and achiral backbone linkers are promising replacements for the phosphodiester linkages of antisense oligonucleotides that target mRNA sequences. Results are presented here for the solution structure elucidation by NMR of an RNA x DNA hybrid duplex, r(GCGCAAAACGCG) x d(CGCGTT-SCH2O-TTGCGC) (designated RIII), containing a 3'-thioformacetal (3'-TFMA) backbone substitution in the DNA strand. The 3'-thioformacetal linker can be accommodated in the hybrid duplex in a conformation that is drastically different from its form in a DNA x DNA duplex but close to that of the canonical A-form helix, reflecting the sequence requirement for hybridization. While the global features of RIII are similar to what are described in the literature, the 3'-TFMA modification drives sugar puckers of the adjacent residues to more C3'-endo-like conformations and causes distortions in related twist angles and helical rises. The helical conformation analyses of each of the two strands and the hybrid duplex enable a clear account of the conformational variability of both the DNA and RNA strands. The A-tract in the RNA strand features an overall straight helix and a more prominent bend at the 3'-end CG step. The structure of RIII provides a structural basis for the improved thermal stability of RIII compared to the corresponding DNA x DNA duplex and insights into the factors that are important concerns for the design of new, effective antisense oligonucleotides.
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(as it appears on PubMed at http://www.pubmed.gov), where 9100003 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9100003}}
==About this Structure==
==About this Structure==
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1AC3 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AC3 OCA].
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1AC3 is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AC3 OCA].
==Reference==
==Reference==
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[[Category: Structure]]
[[Category: Structure]]
[[Category: Thioformacetal backbone modification]]
[[Category: Thioformacetal backbone modification]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:05:17 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 16:33:34 2008''

Revision as of 13:33, 30 June 2008

Image:1ac3.png

Template:STRUCTURE 1ac3

SOLUTION STRUCTURE OF AN RNA-DNA HYBRID DUPLEX CONTAINING A 3'-THIOFORMACETAL LINKER AND AN RNA A-TRACT, NMR, 8 STRUCTURES

Template:ABSTRACT PUBMED 9100003

About this Structure

1AC3 is a Single protein structure. Full experimental information is available from OCA.

Reference

Solution structure of an RNA x DNA hybrid duplex containing a 3'-thioformacetal linker and an RNA A-tract., Cross CW, Rice JS, Gao X, Biochemistry. 1997 Apr 8;36(14):4096-107. PMID:9100003

Page seeded by OCA on Mon Jun 30 16:33:34 2008

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