1acw

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1acw.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1acw.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1acw| PDB=1acw | SCENE= }}
{{STRUCTURE_1acw| PDB=1acw | SCENE= }}
-
'''SOLUTION NMR STRUCTURE OF P01, A NATURAL SCORPION PEPTIDE STRUCTURALLY ANALOGOUS TO SCORPION TOXINS SPECIFIC FOR APAMIN-SENSITIVE POTASSIUM CHANNEL, 25 STRUCTURES'''
+
===SOLUTION NMR STRUCTURE OF P01, A NATURAL SCORPION PEPTIDE STRUCTURALLY ANALOGOUS TO SCORPION TOXINS SPECIFIC FOR APAMIN-SENSITIVE POTASSIUM CHANNEL, 25 STRUCTURES===
-
==Overview==
+
<!--
-
The venom of the North African scorpion Androctonus mauretanicus mauretanicus possesses numerous highly active neurotoxins that specifically bind to various ion channels. One of these, P05, has been found to bind specifically to calcium-activated potassium channels and also to compete with apamin, a toxin extracted from bee venom. Besides the highly potent ones, several of these peptides (including that of P01) have been purified and been found to possess only a very weak, although significant, activity in competition with apamin. The amino acid sequence of P01 shows that it is shorter than P05 by two residues. This deletion occurs within an alpha-helix stretch (residues 5-12). This alpha-helix has been shown to be involved in the interaction of P05 with its receptor via two arginine residues. These two arginines are absent in the P01 sequence. Furthermore, a proline residue in position 7 of the P01 sequence may act as an alpha-helix breaker. We have determined the solution structure of P01 by conventional two-dimensional 1H nuclear magnetic resonance and show that 1) the proline residue does not disturb the alpha-helix running from residues 5 to 12; 2) the two arginines are topologically replaced by two acidic residues, which explains the drop in activity; 3) the residual binding activity may be due to the histidine residue in position 9; and 4) the overall secondary structure is conserved, i.e., an alpha-helix running from residues 5 to 12, two antiparallel stretches of beta-sheet (residues 15-20 and 23-27) connected by a type I' beta-turn, and three disulfide bridges connecting the alpha-helix to the beta-sheet.
+
The line below this paragraph, {{ABSTRACT_PUBMED_8778783}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 8778783 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_8778783}}
==About this Structure==
==About this Structure==
-
1ACW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Androctonus_mauretanicus_mauretanicus Androctonus mauretanicus mauretanicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ACW OCA].
+
1ACW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Androctonus_mauretanicus_mauretanicus Androctonus mauretanicus mauretanicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ACW OCA].
==Reference==
==Reference==
Line 36: Line 40:
[[Category: Scorpion toxin]]
[[Category: Scorpion toxin]]
[[Category: Toxin]]
[[Category: Toxin]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:06:32 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 16:35:32 2008''

Revision as of 13:35, 30 June 2008

Image:1acw.png

Template:STRUCTURE 1acw

SOLUTION NMR STRUCTURE OF P01, A NATURAL SCORPION PEPTIDE STRUCTURALLY ANALOGOUS TO SCORPION TOXINS SPECIFIC FOR APAMIN-SENSITIVE POTASSIUM CHANNEL, 25 STRUCTURES

Template:ABSTRACT PUBMED 8778783

About this Structure

1ACW is a Single protein structure of sequence from Androctonus mauretanicus mauretanicus. Full experimental information is available from OCA.

Reference

Solution structure of P01, a natural scorpion peptide structurally analogous to scorpion toxins specific for apamin-sensitive potassium channel., Blanc E, Fremont V, Sizun P, Meunier S, Van Rietschoten J, Thevand A, Bernassau JM, Darbon H, Proteins. 1996 Mar;24(3):359-69. PMID:8778783

Page seeded by OCA on Mon Jun 30 16:35:32 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1acw"
Personal tools