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1afb

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{{STRUCTURE_1afb| PDB=1afb | SCENE= }}
{{STRUCTURE_1afb| PDB=1afb | SCENE= }}
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'''STRUCTURAL BASIS OF GALACTOSE RECOGNITION IN C-TYPE ANIMAL LECTINS'''
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===STRUCTURAL BASIS OF GALACTOSE RECOGNITION IN C-TYPE ANIMAL LECTINS===
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==Overview==
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The asialoglycoprotein receptors and many other C-type (Ca2+-dependent) animal lectins specifically recognize galactose- or N-acetylgalactosamine-terminated oligosaccharides. Analogous binding specificity can be engineered into the homologous rat mannose-binding protein A by changing three amino acids and inserting a glycine-rich loop (Iobst, S. T., and Drickamer, K. (1994) J. Biol. Chem. 269, 15512-15519). Crystal structures of this mutant complexed with beta-methyl galactoside and N-acetylgalactosamine (GalNAc) reveal that as with wild-type mannose-binding proteins, the 3- and 4-OH groups of the sugar directly coordinate Ca2+ and form hydrogen bonds with amino acids that also serve as Ca2+ ligands. The different stereochemistry of the 3- and 4-OH groups in mannose and galactose, combined with a fixed Ca2+ coordination geometry, leads to different pyranose ring locations in the two cases. The glycine-rich loop provides selectivity against mannose by holding a critical tryptophan in a position optimal for packing with the apolar face of galactose but incompatible with mannose binding. The 2-acetamido substituent of GalNAc is in the vicinity of amino acid positions identified by site-directed mutagenesis (Iobst, S. T., and Drickamer, K. (1996) J. Biol. Chem. 271, 6686-6693) as being important for the formation of a GalNAc-selective binding site.
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(as it appears on PubMed at http://www.pubmed.gov), where 8636086 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8636086}}
==About this Structure==
==About this Structure==
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[[Category: C-type lectin]]
[[Category: C-type lectin]]
[[Category: Calcium-binding protein]]
[[Category: Calcium-binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:11:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 16:43:43 2008''

Revision as of 13:43, 30 June 2008

Image:1afb.png

Template:STRUCTURE 1afb

STRUCTURAL BASIS OF GALACTOSE RECOGNITION IN C-TYPE ANIMAL LECTINS

Template:ABSTRACT PUBMED 8636086

About this Structure

1AFB is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

Structural basis of galactose recognition by C-type animal lectins., Kolatkar AR, Weis WI, J Biol Chem. 1996 Mar 22;271(12):6679-85. PMID:8636086

Page seeded by OCA on Mon Jun 30 16:43:43 2008

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