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1ame

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{{STRUCTURE_1ame| PDB=1ame | SCENE= }}
{{STRUCTURE_1ame| PDB=1ame | SCENE= }}
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'''CRYSTAL STRUCTURE OF TYPE III ANTIFREEZE PROTEIN AT 4 C'''
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===CRYSTAL STRUCTURE OF TYPE III ANTIFREEZE PROTEIN AT 4 C===
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==Overview==
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Fish antifreeze proteins (AFP's) depress the freezing point of blood and other body fluids below that of the surrounding seawater by binding to and inhibiting the growth of seed ice crystals. The high-resolution crystal structure of type III AFP, determined at room temperature, reveals a remarkably flat surface containing most of the ice-binding residues [Jia et al. (1996). Nature (London), 384, 285-288]. Since AFP's function at temperatures close to 273 K, it is important to know whether the structure determined at room temperature undergoes any change at much lower temperature. Therefore, type III AFP has been crystallized at 277 K and its structure determined. Although crystallization conditions at 277 K were similar to those at approximately 295 K, crystal growth took much longer at the lower temperature. Crystals grown at the two temperatures were isomorphous. Initial crystals appeared within 40-50 d and grew to their final size in about 8-12 months, instead of a couple of days at approximately 295 K. The type III antifreeze protein structure from crystals grown at 277 K was essentially the same as that determined at approximately 295 K, with the exception of some minor changes in side-chain conformation. The result is an indication that temperature has a minimal effect on the structure of type III AFP, thus lending increased physiological validity to the room-temperature structure which was used for the initial ice-binding modelling.
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(as it appears on PubMed at http://www.pubmed.gov), where 9761880 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9761880}}
==About this Structure==
==About this Structure==
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[[Category: Crystallization]]
[[Category: Crystallization]]
[[Category: Freezing point]]
[[Category: Freezing point]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:26:53 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 17:08:57 2008''

Revision as of 14:08, 30 June 2008

Image:1ame.png

Template:STRUCTURE 1ame

CRYSTAL STRUCTURE OF TYPE III ANTIFREEZE PROTEIN AT 4 C

Template:ABSTRACT PUBMED 9761880

About this Structure

1AME is a Single protein structure of sequence from Macrozoarces americanus. Full crystallographic information is available from OCA.

Reference

Structure of type III antifreeze protein at 277 K., Ye Q, Leinala E, Jia Z, Acta Crystallogr D Biol Crystallogr. 1998 Jul 1;54(Pt 4):700-2. PMID:9761880

Page seeded by OCA on Mon Jun 30 17:08:57 2008

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