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1b8f

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[[Image:1b8f.gif|left|200px]]
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{{STRUCTURE_1b8f| PDB=1b8f | SCENE= }}
{{STRUCTURE_1b8f| PDB=1b8f | SCENE= }}
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'''HISTIDINE AMMONIA-LYASE (HAL) FROM PSEUDOMONAS PUTIDA'''
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===HISTIDINE AMMONIA-LYASE (HAL) FROM PSEUDOMONAS PUTIDA===
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==Overview==
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Histidine ammonia-lyase (EC 4.3.1.3) catalyzes the nonoxidative elimination of the alpha-amino group of histidine and is closely related to the important plant enzyme phenylalanine ammonia-lyase. The crystal structure of histidase from Pseudomonas putida was determined at 2.1 A resolution revealing a homotetramer with D2 symmetry, the molecular center of which is formed by 20 nearly parallel alpha-helices. The chain fold, but not the sequence, resembles those of fumarase C and related proteins. The structure shows that the reactive electrophile is a 4-methylidene-imidazole-5-one, which is formed autocatalytically by cyclization and dehydration of residues 142-144 with the sequence Ala-Ser-Gly. With respect to the first dehydration step, this modification resembles the chromophore of the green fluorescent protein. The active center is clearly established by the modification and by mutations. The observed geometry allowed us to model the bound substrate at a high confidence level. A reaction mechanism is proposed.
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(as it appears on PubMed at http://www.pubmed.gov), where 10220322 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10220322}}
==About this Structure==
==About this Structure==
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[[Category: Ammonia-lyase]]
[[Category: Ammonia-lyase]]
[[Category: Histidine degradation]]
[[Category: Histidine degradation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:12:11 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 18:33:18 2008''

Revision as of 15:33, 30 June 2008

Image:1b8f.png

Template:STRUCTURE 1b8f

HISTIDINE AMMONIA-LYASE (HAL) FROM PSEUDOMONAS PUTIDA

Template:ABSTRACT PUBMED 10220322

About this Structure

1B8F is a Single protein structure of sequence from Pseudomonas putida. Full crystallographic information is available from OCA.

Reference

Crystal structure of histidine ammonia-lyase revealing a novel polypeptide modification as the catalytic electrophile., Schwede TF, Retey J, Schulz GE, Biochemistry. 1999 Apr 27;38(17):5355-61. PMID:10220322

Page seeded by OCA on Mon Jun 30 18:33:18 2008

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