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1bua

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[[Image:1bua.gif|left|200px]]
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{{STRUCTURE_1bua| PDB=1bua | SCENE= }}
{{STRUCTURE_1bua| PDB=1bua | SCENE= }}
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'''STRUCTURAL AND ENERGETIC ORIGINS OF INDIRECT READOUT IN SITE-SPECIFIC DNA CLEAVAGE BY A RESTRICTION ENDONUCLEASE'''
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===STRUCTURAL AND ENERGETIC ORIGINS OF INDIRECT READOUT IN SITE-SPECIFIC DNA CLEAVAGE BY A RESTRICTION ENDONUCLEASE===
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==Overview==
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Specific recognition by EcoRV endonuclease of its cognate, sharply bent GATATC site at the center TA step occurs solely via hydrophobic interaction with thymine methyl groups. Mechanistic kinetic analyses of base analog-substituted DNAs at this position reveal that direct readout provides 5 kcal mol(-1) toward specificity, with an additional 6-10 kcal mol(-1) arising from indirect readout. Crystal structures of several base analog complexes show that the major-groove hydrophobic contacts are crucial to forming required divalent metal-binding sites, and that indirect readout operates in part through the sequence-dependent free-energy cost of unstacking the center base-pair step of the DNA.
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(as it appears on PubMed at http://www.pubmed.gov), where 10074946 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10074946}}
==About this Structure==
==About this Structure==
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[[Category: Martin, A M.]]
[[Category: Martin, A M.]]
[[Category: Perona, J J.]]
[[Category: Perona, J J.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:57:52 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 19:43:55 2008''

Revision as of 16:43, 30 June 2008

Image:1bua.png

Template:STRUCTURE 1bua

STRUCTURAL AND ENERGETIC ORIGINS OF INDIRECT READOUT IN SITE-SPECIFIC DNA CLEAVAGE BY A RESTRICTION ENDONUCLEASE

Template:ABSTRACT PUBMED 10074946

About this Structure

1BUA is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structural and energetic origins of indirect readout in site-specific DNA cleavage by a restriction endonuclease., Martin AM, Sam MD, Reich NO, Perona JJ, Nat Struct Biol. 1999 Mar;6(3):269-77. PMID:10074946

Page seeded by OCA on Mon Jun 30 19:43:55 2008

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