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1cem

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{{STRUCTURE_1cem| PDB=1cem | SCENE= }}
{{STRUCTURE_1cem| PDB=1cem | SCENE= }}
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'''ENDOGLUCANASE A (CELA) CATALYTIC CORE, RESIDUES 33-395'''
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===ENDOGLUCANASE A (CELA) CATALYTIC CORE, RESIDUES 33-395===
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==Overview==
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BACKGROUND. Cellulases, which catalyze the hydrolysis of glycosidic bonds in cellulose, can be classified into several different protein families. Endoglucanase CelA is a member of glycosyl hydrolase family 8, a family for which no structural information was previously available. RESULTS. The crystal structure of CelA was determined by multiple isomorphous replacement and refined to 1.65 A resolution. The protein folds into a regular (alpha/alpha)6 barrel formed by six inner and six outer alpha helices. Cello-oligosaccharides bind to an acidic cleft containing at least five D-glucosyl-binding subsites (A-E) such that the scissile glycosidic linkage lies between subsites C and D. The strictly conserved residue Glu95, which occupies the center of the substrate-binding cleft and is hydrogen bonded to the glycosidic oxygen, has been assigned the catalytic role of proton donor. CONCLUSIONS. The present analysis provides a basis for modeling homologous family 8 cellulases. The architecture of the active-site cleft, presenting at least five glucosyl-binding subsites, explains why family 8 cellulases cleave cello-oligosaccharide polymers that are at least five D-glycosyl subunits long. Furthermore, the structure of CelA allows comparison with (alpha/alpha)6 barrel glycosidases that are not related in sequence, suggesting a possible, albeit distant, evolutionary relationship between different families of glycosyl hydrolases.
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(as it appears on PubMed at http://www.pubmed.gov), where 8805535 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8805535}}
==About this Structure==
==About this Structure==
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[[Category: Glycosyl hydrolase]]
[[Category: Glycosyl hydrolase]]
[[Category: Glycosyltransferase]]
[[Category: Glycosyltransferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:38:56 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:38:31 2008''

Revision as of 17:38, 30 June 2008

Image:1cem.png

Template:STRUCTURE 1cem

ENDOGLUCANASE A (CELA) CATALYTIC CORE, RESIDUES 33-395

Template:ABSTRACT PUBMED 8805535

About this Structure

1CEM is a Single protein structure of sequence from Clostridium thermocellum. Full crystallographic information is available from OCA.

Reference

The crystal structure of endoglucanase CelA, a family 8 glycosyl hydrolase from Clostridium thermocellum., Alzari PM, Souchon H, Dominguez R, Structure. 1996 Mar 15;4(3):265-75. PMID:8805535

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