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1cf3

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[[Image:1cf3.gif|left|200px]]
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{{STRUCTURE_1cf3| PDB=1cf3 | SCENE= }}
{{STRUCTURE_1cf3| PDB=1cf3 | SCENE= }}
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'''GLUCOSE OXIDASE FROM APERGILLUS NIGER'''
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===GLUCOSE OXIDASE FROM APERGILLUS NIGER===
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==Overview==
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Glucose oxidase is a flavin-dependent enzyme which catalyses the oxidation of beta-D-glucose by molecular oxygen to delta-gluconolactone and hydrogen peroxide. The structure of the enzyme from Aspergillus niger, previously refined at 2.3 A resolution, has been refined at 1.9 A resolution to an R value of 19.0%, and the structure of the enzyme from Penicillium amagasakiense, which has 65% sequence identity, has been determined by molecular replacement and refined at 1.8 A resolution to an R value of 16.4%. The structures of the partially deglycosylated enzymes have an r.m.s. deviation of 0.7 A for main-chain atoms and show four N-glycosylation sites, with an extended carbohydrate moiety at Asn89. Substrate complexes of the enzyme from A. niger were modelled by force-field methods. The resulting model is consistent with results from site-directed mutagenesis experiments and shows the beta-D-glucose molecule in the active site of glucose oxidase, stabilized by 12 hydrogen bonds and by hydrophobic contacts to three neighbouring aromatic residues and to flavin adenine dinucleotide. Other hexoses, such as alpha-D-glucose, mannose and galactose, which are poor substrates for the enzyme, and 2-deoxy-D-glucose, form either fewer bonds or unfavourable contacts with neighbouring amino acids. Simulation of the complex between the reduced enzyme and the product, delta-gluconolactone, has provided an explanation for the lack of product inhibition by the lactone.
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(as it appears on PubMed at http://www.pubmed.gov), where 10216293 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10216293}}
==About this Structure==
==About this Structure==
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[[Category: Hecht, H J.]]
[[Category: Hecht, H J.]]
[[Category: Kalisz, H.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:39:39 2008''

Revision as of 17:39, 30 June 2008

Image:1cf3.png

Template:STRUCTURE 1cf3

GLUCOSE OXIDASE FROM APERGILLUS NIGER

Template:ABSTRACT PUBMED 10216293

About this Structure

1CF3 is a Single protein structure of sequence from Aspergillus niger. Full crystallographic information is available from OCA.

Reference

1.8 and 1.9 A resolution structures of the Penicillium amagasakiense and Aspergillus niger glucose oxidases as a basis for modelling substrate complexes., Wohlfahrt G, Witt S, Hendle J, Schomburg D, Kalisz HM, Hecht HJ, Acta Crystallogr D Biol Crystallogr. 1999 May;55(Pt 5):969-77. PMID:10216293

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