1dfu

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{{STRUCTURE_1dfu| PDB=1dfu | SCENE= }}
{{STRUCTURE_1dfu| PDB=1dfu | SCENE= }}
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'''CRYSTAL STRUCTURE OF E.COLI RIBOSOMAL PROTEIN L25 COMPLEXED WITH A 5S RRNA FRAGMENT AT 1.8 A RESOLUTION'''
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===CRYSTAL STRUCTURE OF E.COLI RIBOSOMAL PROTEIN L25 COMPLEXED WITH A 5S RRNA FRAGMENT AT 1.8 A RESOLUTION===
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==Overview==
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The crystal structure of Escherichia coli ribosomal protein L25 bound to an 18-base pair portion of 5S ribosomal RNA, which contains "loop E," has been determined at 1.8-A resolution. The protein primarily recognizes a unique RNA shape, although five side chains make direct or water-mediated interactions with bases. Three beta-strands lie in the widened minor groove of loop E formed by noncanonical base pairs and cross-strand purine stacks, and an alpha-helix interacts in an adjacent widened major groove. The structure of loop E is largely the same as that of uncomplexed RNA (rms deviation of 0.4 A for 11 base pairs), and 3 Mg(2+) ions that stabilize the noncanonical base pairs lie in the same or similar locations in both structures. Perhaps surprisingly, those residues interacting with the RNA backbone are the most conserved among known L25 sequences, whereas those interacting with the bases are not.
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(as it appears on PubMed at http://www.pubmed.gov), where 10696113 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10696113}}
==About this Structure==
==About this Structure==
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[[Category: Steitz, T A.]]
[[Category: Steitz, T A.]]
[[Category: Protein-rna complex]]
[[Category: Protein-rna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 23:01:02 2008''

Revision as of 20:01, 30 June 2008

Image:1dfu.png

Template:STRUCTURE 1dfu

CRYSTAL STRUCTURE OF E.COLI RIBOSOMAL PROTEIN L25 COMPLEXED WITH A 5S RRNA FRAGMENT AT 1.8 A RESOLUTION

Template:ABSTRACT PUBMED 10696113

About this Structure

1DFU is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structure of Escherichia coli ribosomal protein L25 complexed with a 5S rRNA fragment at 1.8-A resolution., Lu M, Steitz TA, Proc Natl Acad Sci U S A. 2000 Feb 29;97(5):2023-8. PMID:10696113

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