1eb0

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{{STRUCTURE_1eb0| PDB=1eb0 | SCENE= }}
{{STRUCTURE_1eb0| PDB=1eb0 | SCENE= }}
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'''CRYSTAL STRUCTURE OF BACILLUS PASTEURII UREE AT 1.85 A, PHASED BY SIRAS. TYPE I CRYSTAL FORM.'''
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===CRYSTAL STRUCTURE OF BACILLUS PASTEURII UREE AT 1.85 A, PHASED BY SIRAS. TYPE I CRYSTAL FORM.===
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==Overview==
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Bacillus pasteurii UreE (BpUreE) is a putative chaperone assisting the insertion of Ni(2+) ions in the active site of urease. The x-ray structure of the protein has been determined for two crystal forms, at 1.7 and 1.85 A resolution, using SIRAS phases derived from a Hg(2+)-derivative. BpUreE is composed of distinct N- and C-terminal domains, connected by a short flexible linker. The structure reveals the topology of an elongated homodimer, formed by interaction of the two C-terminal domains through hydrophobic interactions. A single Zn(2+) ion bound to four conserved His-100 residues, one from each monomer, connects two dimers resulting in a tetrameric BpUreE known to be formed in concentrated solutions. The Zn(2+) ion can be replaced by Ni(2+) as shown by anomalous difference maps obtained on a crystal of BpUreE soaked in a solution containing NiCl(2). A large hydrophobic patch surrounding the metal ion site is surface-exposed in the biologically relevant dimer. The BpUreE structure represents the first for this class of proteins and suggests a possible role for UreE in the urease nickel-center assembly.
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(as it appears on PubMed at http://www.pubmed.gov), where 11602602 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11602602}}
==About this Structure==
==About this Structure==
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[[Category: Putative ni-chaperone]]
[[Category: Putative ni-chaperone]]
[[Category: Urease accessory protein]]
[[Category: Urease accessory protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:25:59 2008''

Revision as of 21:26, 30 June 2008

Image:1eb0.png

Template:STRUCTURE 1eb0

CRYSTAL STRUCTURE OF BACILLUS PASTEURII UREE AT 1.85 A, PHASED BY SIRAS. TYPE I CRYSTAL FORM.

Template:ABSTRACT PUBMED 11602602

About this Structure

1EB0 is a Single protein structure of sequence from Sporosarcina pasteurii. Full crystallographic information is available from OCA.

Reference

Structural basis for Ni(2+) transport and assembly of the urease active site by the metallochaperone UreE from Bacillus pasteurii., Remaut H, Safarov N, Ciurli S, Van Beeumen J, J Biol Chem. 2001 Dec 28;276(52):49365-70. Epub 2001 Oct 15. PMID:11602602

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