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1ecl

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[[Image:1ecl.gif|left|200px]]
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{{STRUCTURE_1ecl| PDB=1ecl | SCENE= }}
{{STRUCTURE_1ecl| PDB=1ecl | SCENE= }}
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'''AMINO TERMINAL 67KDA DOMAIN OF ESCHERICHIA COLI DNA TOPOISOMERASE I (RESIDUES 2-590 OF MATURE PROTEIN) CLONING ARTIFACT ADDS TWO RESIDUES TO THE AMINO-TERMINUS WHICH WERE NOT OBSERVED IN THE EXPERIMENTAL ELECTRON DENSITY (GLY-2, SER-1).'''
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===AMINO TERMINAL 67KDA DOMAIN OF ESCHERICHIA COLI DNA TOPOISOMERASE I (RESIDUES 2-590 OF MATURE PROTEIN) CLONING ARTIFACT ADDS TWO RESIDUES TO THE AMINO-TERMINUS WHICH WERE NOT OBSERVED IN THE EXPERIMENTAL ELECTRON DENSITY (GLY-2, SER-1).===
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==Overview==
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The three-dimensional structure of the 67K amino-terminal fragment of Escherichia coli DNA topoisomerase I has been determined to 2.2 A resolution. The polypeptide folds in an unusual way to give four distinct domains enclosing a hole large enough to accommodate a double-stranded DNA. The active-site tyrosyl residue, which is involved in the transient breakage of a DNA strand and the formation of a covalent enzyme-DNA intermediate, is present at the interface of two domains. The structure suggests a plausible mechanism by which E. coli DNA topoisomerase I and other members of the same DNA topoisomerase subfamily could catalyse the passage of one DNA strand through a transient break in another strand.
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The line below this paragraph, {{ABSTRACT_PUBMED_8114910}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 8114910 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8114910}}
==About this Structure==
==About this Structure==
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[[Category: Dna cleavage]]
[[Category: Dna cleavage]]
[[Category: Strand passage]]
[[Category: Strand passage]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:56:13 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:29:52 2008''

Revision as of 21:29, 30 June 2008

Image:1ecl.png

Template:STRUCTURE 1ecl

AMINO TERMINAL 67KDA DOMAIN OF ESCHERICHIA COLI DNA TOPOISOMERASE I (RESIDUES 2-590 OF MATURE PROTEIN) CLONING ARTIFACT ADDS TWO RESIDUES TO THE AMINO-TERMINUS WHICH WERE NOT OBSERVED IN THE EXPERIMENTAL ELECTRON DENSITY (GLY-2, SER-1).

Template:ABSTRACT PUBMED 8114910

About this Structure

1ECL is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Three-dimensional structure of the 67K N-terminal fragment of E. coli DNA topoisomerase I., Lima CD, Wang JC, Mondragon A, Nature. 1994 Jan 13;367(6459):138-46. PMID:8114910

Page seeded by OCA on Tue Jul 1 00:29:52 2008

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