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1ed9

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{{STRUCTURE_1ed9| PDB=1ed9 | SCENE= }}
{{STRUCTURE_1ed9| PDB=1ed9 | SCENE= }}
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'''STRUCTURE OF E. COLI ALKALINE PHOSPHATASE WITHOUT THE INORGANIC PHOSPHATE AT 1.75A RESOLUTION'''
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===STRUCTURE OF E. COLI ALKALINE PHOSPHATASE WITHOUT THE INORGANIC PHOSPHATE AT 1.75A RESOLUTION===
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==Overview==
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Here, X-ray crystallography has been used to investigate the proposed double in-line displacement mechanism of Escherichia coli alkaline phosphatase in which two of the three active-site metal ions have a direct role in catalysis. Two new X-ray crystal structures of the wild-type enzyme in the absence and presence of inorganic phosphate have been refined at 1.75 A to final working R-factors of 15.4% and 16.4%, respectively. In the refinement of both structures, residues in the active sites were treated anisotropically. The ellipsoids resulting from the partial anisotropic refinement show a clear route for the binding and release of substrate/product. In addition, a direct comparison of the refined structures with and without phosphate reveal a strong correlation between the occupancy of the third metal-binding site and the conformation of the Ser102 nucleophile. These findings clarify two important and unresolved aspects of the previously proposed catalytic mechanism, how Ser102 is activated for nucleophilic attack and why a magnesium ion in the third metal site is required for catalysis. Analysis of these results suggest that three metal-ion assisted catalysis is a more accurate description of the mechanism of the alkaline phosphatase reaction. A revised mechanism for the catalytic reaction of alkaline phosphatase is proposed on the basis of the two new X-ray crystal structures reported.
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The line below this paragraph, {{ABSTRACT_PUBMED_10873454}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 10873454 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10873454}}
==About this Structure==
==About this Structure==
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[[Category: Free of phosphate]]
[[Category: Free of phosphate]]
[[Category: Wild type]]
[[Category: Wild type]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:57:28 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:31:20 2008''

Revision as of 21:31, 30 June 2008

Image:1ed9.png

Template:STRUCTURE 1ed9

STRUCTURE OF E. COLI ALKALINE PHOSPHATASE WITHOUT THE INORGANIC PHOSPHATE AT 1.75A RESOLUTION

Template:ABSTRACT PUBMED 10873454

About this Structure

1ED9 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

A revised mechanism for the alkaline phosphatase reaction involving three metal ions., Stec B, Holtz KM, Kantrowitz ER, J Mol Biol. 2000 Jun 23;299(5):1303-11. PMID:10873454

Page seeded by OCA on Tue Jul 1 00:31:20 2008

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