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1egk

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{{STRUCTURE_1egk| PDB=1egk | SCENE= }}
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'''CRYSTAL STRUCTURE OF A NUCLEIC ACID FOUR-WAY JUNCTION'''
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===CRYSTAL STRUCTURE OF A NUCLEIC ACID FOUR-WAY JUNCTION===
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==Overview==
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A crystal structure of a 108 nucleotide RNA-DNA complex containing a four-way junction was solved at 3.1 A resolution. The structure of the junction differs substantially from the "stacked-X" conformation observed previously, due to a 135 degrees rotation of the branches. Comparison of the two conformers provides insight into the factors contributing to the flexibility of four-way junctions. The stacked-X conformation maximizes base-stacking but causes unfavorable repulsion between phosphate groups, whereas the 135 degrees -rotated "crossed" conformation minimizes electrostatic clashes at the expense of reduced base-stacking. Despite the large rotation of the branches, both junction structures exhibit an antiparallel arrangement of the continuous strands and opposite polarity of the crossover strands.
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(as it appears on PubMed at http://www.pubmed.gov), where 10864501 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10864501}}
==About this Structure==
==About this Structure==
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[[Category: Ribozyme]]
[[Category: Ribozyme]]
[[Category: Rna-dna complex]]
[[Category: Rna-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:04:33 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:39:57 2008''

Revision as of 21:39, 30 June 2008

Image:1egk.png

Template:STRUCTURE 1egk

CRYSTAL STRUCTURE OF A NUCLEIC ACID FOUR-WAY JUNCTION

Template:ABSTRACT PUBMED 10864501

About this Structure

Full crystallographic information is available from OCA.

Reference

Alternative conformations of a nucleic acid four-way junction., Nowakowski J, Shim PJ, Stout CD, Joyce GF, J Mol Biol. 2000 Jun 30;300(1):93-102. PMID:10864501

Page seeded by OCA on Tue Jul 1 00:39:57 2008

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